Fujimoto Y, Nakatani E, Minamino H, Takahashi M, Sakuma S, Fujita T
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
Biochim Biophys Acta. 1990 May 1;1044(1):65-9. doi: 10.1016/0005-2760(90)90219-n.
Intact slice preparations of rabbit stomach (antral mucosa, corporal mucosa, antral muscle and corporal muscle) were incubated and the released prostaglandins (PGs) were measured by reverse-phase high-performance liquid chromatography using 9-anthryldiazomethane for derivatization. With respect to total PG production, the highest amounts were generated by antral mucosal slices. Antral mucosal slices produced PGE2, 6-keto PGF1 alpha, thromboxane B2, PGF2 alpha and PGD2 (in descending order of magnitude) and possessed a high capacity for producing 13,14-dihydro-15-keto derivatives of both PGE2 and PGF2 alpha. Studies utilizing aspirin, EGTA or Ca2+ revealed that PG release by antral mucosal slices in the present in vitro system reflects a composite of the activities of phospholipase A3, PG cyclooxygenase and PG-metabolizing enzymes. These results show that antral mucosal slices will be useful in physiological and pharmacological studies on PG synthesis and metabolism of the stomach.
将兔胃的完整切片标本（胃窦黏膜、胃体黏膜、胃窦肌层和胃体肌层）进行孵育，并用9-蒽基重氮甲烷进行衍生化，通过反相高效液相色谱法测定释放的前列腺素（PGs）。就总PG生成量而言，胃窦黏膜切片产生的量最高。胃窦黏膜切片产生PGE2、6-酮-PGF1α、血栓素B2、PGF2α和PGD2（按含量递减顺序排列），并且具有产生PGE2和PGF2α的13,14-二氢-15-酮衍生物的高能力。利用阿司匹林、乙二醇双（2-氨基乙基醚）四乙酸（EGTA）或Ca2+进行的研究表明，在当前体外系统中胃窦黏膜切片释放PG反映了磷脂酶A3、PG环氧化酶和PG代谢酶活性的综合作用。这些结果表明，胃窦黏膜切片将有助于进行关于胃PG合成和代谢的生理学和药理学研究。
