Ota H, Fukushima M, Maki M
Tohoku J Exp Med. 1987 Jan;151(1):33-40. doi: 10.1620/tjem.151.33.
The critical role of high level of serum LH in polycystic ovary syndrome was evaluated in rats by the long-term administration of hCG. Wistar-Imamichi strain mature female rats (age; 12 weeks) which showed at least two consecutive estrous cycles in vaginal smears were daily injected 10 IU hCG subcutaneously from diestrus for 80 days. Control rats were received saline solutions. On the next day after the last administration the rats were killed, and serum hormone levels and histological changes in the ovaries were examined. In 8 of 11 control rats the vaginal smears showed the regular estrous cycles (group 1) during the experimental period. None of the controls exhibited polycystic ovaries. In 19 of 25 experimented rats there were old corpora lutea and degenerated follicles (group 2). The remained animals (n = 6; group 3) showed polycystic ovaries and no corpora lutea except one. HCG treatment elevated the serum prolactin and estradiol levels in group 3, but reduced the progesterone level. Thus, it was suggested that the hCG-induced formation of PCO in rats might be able to refer to the pathogenesis in polycystic ovary syndrome.
通过长期给予人绒毛膜促性腺激素（hCG），在大鼠中评估了高水平血清促黄体生成素（LH）在多囊卵巢综合征中的关键作用。对Wistar-Imamichi品系成熟雌性大鼠（年龄：12周）进行阴道涂片检查，显示至少有两个连续发情周期，从间情期开始每天皮下注射10国际单位hCG，持续80天。对照组大鼠注射生理盐水。在最后一次给药后的第二天处死大鼠，检查血清激素水平和卵巢的组织学变化。11只对照大鼠中有8只在实验期间阴道涂片显示有规律的发情周期（第1组）。所有对照组均未出现多囊卵巢。25只实验大鼠中有19只存在陈旧性黄体和退化卵泡（第2组）。其余动物（n = 6；第3组）除一只外均显示多囊卵巢且无黄体。hCG治疗使第3组大鼠血清催乳素和雌二醇水平升高，但降低了孕酮水平。因此，提示hCG诱导大鼠多囊卵巢的形成可能与多囊卵巢综合征的发病机制有关。
