Ota H, Wakizaka A, Fukushima M, Maki M
Tohoku J Exp Med. 1986 Mar;148(3):313-25. doi: 10.1620/tjem.148.313.
To establish the role of hormone receptors in patients with polycystic ovary (PCO), PCO rats were prepared by treating with testosterone propionate (TP). Five-day old immature female rats were subcutaneously injected with 1.25 mg TP in sesame oil. They were then killed at the age of 12 weeks. The ovarian receptors for LH and FSH as well as serum hormone levels were investigated in PCO rats and also in control rats at the various stages of the estrous cycle. The LH receptor binding in the TP-treated ovaries was elevated almost as high as that of proestrus control, and was observed to be higher than the other control. The FSH receptor binding of PCO rats was elevated to 173% of that of diestrus control, which showed the highest value throughout the cycle. Thus, the gonadotropin receptors in PCO rats appeared to be in an activated state. High levels of the receptor binding were due to an increase in receptor binding sites. Serum LH level was significantly higher than that of diestrus control but still remained lower than that of proestrus control. In contrast, FSH level was as low as that of diestrus control. Prolactin level was markedly elevated and 17- and 2-fold higher than that of diestrus and proestrus control, respectively. Estradiol level was higher than that of diestrus control, increasing to almost the same level during proestrus control. While progesterone level was largely depressed to 23 and 13% of that of diestrus and proestrus control, respectively, testosterone level was almost the same as that of diestrus control. From these results, it was suggested that tonic secretion of LH, low level of FSH, and markedly high levels of prolactin would increase the gonadotropin receptors and result in extremely low production of progesterone in rat ovaries. Clinically, elevated levels of the LH and FSH receptors may be a relevant occurrence in PCO patients.
为确定激素受体在多囊卵巢（PCO）患者中的作用，通过丙酸睾酮（TP）处理制备了PCO大鼠。5日龄未成熟雌性大鼠皮下注射1.25mg溶于芝麻油的TP。然后在12周龄时将它们处死。研究了PCO大鼠以及处于发情周期各个阶段的对照大鼠的卵巢LH和FSH受体以及血清激素水平。TP处理的卵巢中LH受体结合几乎升高到与动情前期对照相同的水平，并且观察到高于其他对照。PCO大鼠的FSH受体结合升高到动情后期对照的173%，这是整个周期中的最高值。因此，PCO大鼠中的促性腺激素受体似乎处于激活状态。受体结合水平高是由于受体结合位点增加。血清LH水平显著高于动情后期对照，但仍低于动情前期对照。相比之下，FSH水平与动情后期对照一样低。催乳素水平显著升高，分别比动情后期和动情前期对照高17倍和2倍。雌二醇水平高于动情后期对照，在动情前期对照期间几乎增加到相同水平。而孕酮水平分别大幅降低至动情后期和动情前期对照的23%和13%，睾酮水平与动情后期对照几乎相同。从这些结果表明，LH的持续性分泌、FSH的低水平以及催乳素的显著高水平会增加促性腺激素受体，并导致大鼠卵巢中孕酮的产生极低。临床上，LH和FSH受体水平升高可能是PCO患者的一个相关现象。
