Russell Scott M, Fletcher Daniel L, Cox Nelson A
Department of Poultry Science, University of Georgia, Athens, Georgia 30602.
USDA, ARS, Richard B. Russell Agricultural Research Center, Athens, Georgia 30613.
J Food Prot. 1992 Mar;55(3):167-170. doi: 10.4315/0362-028X-55.3.167.
Broiler chicken carcasses were obtained from a commercial processing facility, rinsed with 100 ml of sterile brain heart infusion broth and the solutions held at 3, 4, 5, 6, or 9°C for 10 d. At 0, 7, and 10 d, total plate counts (TPC) and impedimetric measurements were conducted at incubation temperatures of 18 and 42°C. For plates incubated at 42°C, counts did not change after 7 d of storage when broiler carcass rinses were held at 3 and 4°C. When held at 5, 6, and 9°C, however, plate counts increased significantly. Impedimetric detection times did not change when rinses were held at 3, 4, and 5°C but decreased significantly for rinses stored at 6 or 9°C. Upon incubation at 18°C, TPCs increased and impedimetric detection times decreased significantly when carcass rinses were held at 3, 4, 5, 6, and 9°C. Mixed bacterial flora recovered from fresh broilers and incubated at 42°C do not grow in storage temperatures below 5°C as determined by TPC and 6°C as determined by impedance analysis. Such a model system may be applicable to practical food handling systems to determine if products were held above a given temperature.
肉鸡胴体取自一家商业加工设施，用100毫升无菌脑心浸液肉汤冲洗，然后将溶液分别在3、4、5、6或9℃下保存10天。在第0、7和10天，分别在18℃和42℃的培养温度下进行总平板计数（TPC）和电阻抗测量。对于在42℃下培养的平板，当肉鸡胴体冲洗液在3℃和4℃下保存时，储存7天后菌数没有变化。然而，当在5℃、6℃和9℃下保存时，平板菌数显著增加。当冲洗液在3℃、4℃和5℃下保存时，电阻抗检测时间没有变化，但在6℃或9℃下保存的冲洗液，其电阻抗检测时间显著缩短。当胴体冲洗液在3℃、4℃、5℃、6℃和9℃下保存，并在18℃下培养时，TPC增加，电阻抗检测时间显著缩短。通过TPC测定，从新鲜肉鸡中分离并在42℃下培养的混合细菌菌群，在低于5℃的储存温度下不生长；通过阻抗分析测定，在低于6℃的储存温度下不生长。这样的模型系统可能适用于实际食品处理系统，以确定产品是否在给定温度以上保存过。
