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日本青鳉鳃部 AQP1 和 AQP3 的差异表达和定位 ()。

Differential Expression and Localization of Branchial AQP1 and AQP3 in Japanese Medaka ().

机构信息

Department of Biological Sciences, J. William Fulbright College of Arts and Sciences, University of Arkansas, Fayetteville, AR 72701, USA.

Department of Biology, Natural Science Faculty, University of Southern Denmark, 5230 Odense, Denmark.

出版信息

Cells. 2019 May 8;8(5):422. doi: 10.3390/cells8050422.

DOI:10.3390/cells8050422
PMID:31072010
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6562476/
Abstract

Aquaporins (AQPs) facilitate transmembrane water and solute transport, and in addition to contributing to transepithelial water transport, they safeguard cell volume homeostasis. This study examined the expression and localization of AQP1 and AQP3 in the gills of Japanese medaka ( in response to osmotic challenges and osmoregulatory hormones, cortisol, and prolactin (PRL). mRNA was inversely regulated in response to salinity with high levels in ion-poor water (IPW), intermediate levels in freshwater (FW), and low levels in seawater (SW). AQP3 protein levels decreased upon SW acclimation. By comparison, AQP1 expression was unaffected by salinity. In ex vivo gill incubation experiments, mRNA was stimulated by PRL in a time- and dose-dependent manner but was unaffected by cortisol. In contrast, was unaffected by both PRL and cortisol. Confocal microscopy revealed that AQP3 was abundant in the periphery of gill filament epithelial cells and co-localized at low intensity with Na,K-ATPase in ionocytes. AQP1 was present at a very low intensity in most filament epithelial cells and red blood cells. No epithelial cells in the gill lamellae showed immunoreactivity to AQP3 or AQP1. We suggest that both AQPs contribute to cellular volume regulation in the gill epithelium and that AQP3 is particularly important under hypo-osmotic conditions, while expression of AQP1 is constitutive.

摘要

水通道蛋白 (AQP) 促进跨膜水和溶质转运，除了有助于跨上皮水转运外，还能维持细胞体积稳态。本研究探讨了日本青鳉鳃中 AQP1 和 AQP3 的表达和定位（in response to osmotic challenges and osmoregulatory hormones, cortisol, and prolactin (PRL). ）。mRNA 水平对盐度呈反向调节，在低盐度水中（IPW）表达水平较高，在淡水（FW）中表达水平中等，在海水（SW）中表达水平较低。AQP3 蛋白水平在适应海水时下降。相比之下，AQP1 的表达不受盐度影响。在离体鳃孵育实验中，PRL 以时间和剂量依赖的方式刺激 mRNA 的表达，但不受皮质醇影响。相反，PRL 和皮质醇对 均无影响。共聚焦显微镜显示，AQP3 在鳃丝上皮细胞的周边丰富，并在离子细胞中与 Na,K-ATPase 低强度共定位。AQP1 在大多数鳃丝上皮细胞和红细胞中表达强度非常低。在鳃片上皮细胞中，没有细胞对 AQP3 或 AQP1 表现出免疫反应性。我们认为，这两种 AQP 都有助于鳃上皮细胞的细胞体积调节，而 AQP3 在低渗条件下尤为重要，而 AQP1 的表达则是组成性的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/0c66d040bcde/cells-08-00422-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/0c97e118e526/cells-08-00422-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/661b010960a6/cells-08-00422-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/3093cbfbe2f3/cells-08-00422-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/425ebf9a6731/cells-08-00422-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/7dd20e5d2fff/cells-08-00422-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/41d8b5048d60/cells-08-00422-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/482ea72bf219/cells-08-00422-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/9cbf88beb589/cells-08-00422-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/22069fb3e1ed/cells-08-00422-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/a8847619eebf/cells-08-00422-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/d24478934699/cells-08-00422-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/0c66d040bcde/cells-08-00422-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/0c97e118e526/cells-08-00422-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/661b010960a6/cells-08-00422-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/3093cbfbe2f3/cells-08-00422-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/99c0434f73d9/cells-08-00422-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/425ebf9a6731/cells-08-00422-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/7dd20e5d2fff/cells-08-00422-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/41d8b5048d60/cells-08-00422-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/482ea72bf219/cells-08-00422-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/9cbf88beb589/cells-08-00422-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/22069fb3e1ed/cells-08-00422-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/a8847619eebf/cells-08-00422-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/d24478934699/cells-08-00422-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95ab/6562476/0c66d040bcde/cells-08-00422-g013.jpg

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