[淋球菌在营养肉汤中的培养]
[Cultivation of the gonococcus in nutrient broths].
作者信息
Osmanova M M, Bednova V N, Dmitriev G A, Raskin B M, Osmanov S K
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 1987 Feb(2):3-7.
The dynamics of the multiplication of gonococci and the parameters of their growth have been studied in the process of batch cultivation in liquid culture media with different content of bovine blood serum. The protective and stimulating action of the serum on the growth of gonococci has been shown. The optimization of the process of cultivation has been carried out; as a result, the growth of test strains in a culture medium containing no serum has been achieved. Phasic changes in the ultrastructure of gonococci in the process of their cultivation in liquid media have been followed. 9- to 12-hour cultures of gonococci grown in liquid culture media have been found the most valuable and physiologically active.
在含有不同牛血清含量的液体培养基分批培养过程中，研究了淋球菌繁殖的动力学及其生长参数。已表明血清对淋球菌生长具有保护和刺激作用。已对培养过程进行了优化；结果，实现了测试菌株在无血清培养基中的生长。跟踪了淋球菌在液体培养基中培养过程中超微结构的阶段性变化。已发现，在液体培养基中培养9至12小时的淋球菌培养物最有价值且生理活性最强。
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