Skliar T V, Krysenko A V, Gavriliuk V G, Vinnikov A I
Dniepropetrovsk State University.
Mikrobiol Z. 1998 Jan-Feb;60(1):25-30.
The growth parameters of gonococcus and staphylococcus strains in liquid medium were determined. The generation time varied within the limits from 0.8 to 1.3 h for gonococcus and from 0.47 to 0.60 h for staphylococcus, the specific growth rate varied from 0.51 to 0.91 h-1 for gonococcus and from 1.68 to 2.11 h-1 for staphylococcus, depending on the concentration of sodium humate and bovine serum in the medium. The growth characteristics of analysed staphylococcus strains did not depend on the presence of plasmids. The gonococcal cultures grown in the liquid nutrient medium containing 20% of bovine serum for 8-11 h were the most physiologically active. Sodium humate added to the medium for cultivation in concentration 0.01% stimulated the growth of N. gonorrhoeae.
测定了淋球菌和葡萄球菌菌株在液体培养基中的生长参数。淋球菌的代时在0.8至1.3小时范围内变化，葡萄球菌的代时在0.47至0.60小时范围内变化，淋球菌的比生长速率在0.51至0.91 h⁻¹之间变化，葡萄球菌的比生长速率在1.68至2.11 h⁻¹之间变化，这取决于培养基中腐殖酸钠和牛血清的浓度。所分析的葡萄球菌菌株的生长特性不依赖于质粒的存在。在含有20%牛血清的液体营养培养基中培养8至11小时的淋球菌培养物生理活性最高。添加到培养基中浓度为0.01%的腐殖酸钠刺激了淋病奈瑟菌的生长。
