Department of Urology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, China.
Andrologia. 2019 Sep;51(8):e13302. doi: 10.1111/and.13302. Epub 2019 May 10.
Chronic prostatitis/chronic pelvic pain syndrome (CP⁄CPPS) is the prostate gland inflammation characterised as genitourinary pain in the pelvic region. The rat experimental autoimmune prostatitis (EAP) was achieved to mimic CP⁄CPPS. The expressions of transient receptor potential vanilloid 1 (TRPV1) in the prostate, bladder and spinal dorsal root ganglion (DRG) were analysed by Western blotting. Tropomyosin receptor kinase A (TrkA) and nerve growth factor (NGF) in the DRG were also analysed by Western blotting. Measurements of inflammatory cytokines were carried out according to the instructions of the corresponding kits. The expressions of TRPV1 in the prostate, bladder and DRG in the EAP group were significantly higher than those in the control group. The expressions of NGF and TrkA in the DRG in the EAP group were significantly higher than those in the control group. The levels of serum TNF-α and IL-1β in the EAP group were significantly higher than those in the control group. We conclude that CP⁄CPPS may participate in the pathological activation of neurons in the L5-S1 segment of DRG by activating NGF-TrkA pathway and cause pelvic organ cross-sensitisation by upregulating the expression of TRPV1 in the prostate, bladder and DRG.
慢性前列腺炎/慢性骨盆疼痛综合征（CP/CPPS）是一种前列腺炎症，表现为骨盆区域的泌尿生殖系统疼痛。通过建立大鼠实验性自身免疫性前列腺炎（EAP）来模拟 CP/CPPS。通过 Western blot 分析前列腺、膀胱和脊髓背根神经节（DRG）中瞬时受体电位香草酸 1（TRPV1）的表达。通过 Western blot 分析 DRG 中的原肌球蛋白受体激酶 A（TrkA）和神经生长因子（NGF）。根据相应试剂盒的说明进行炎性细胞因子的测量。EAP 组前列腺、膀胱和 DRG 中 TRPV1 的表达明显高于对照组。EAP 组 DRG 中 NGF 和 TrkA 的表达明显高于对照组。EAP 组血清 TNF-α和 IL-1β水平明显高于对照组。我们得出结论，CP/CPPS 可能通过激活 NGF-TrkA 通路参与 L5-S1 节段 DRG 神经元的病理性激活，并通过上调前列腺、膀胱和 DRG 中 TRPV1 的表达引起盆腔器官交叉敏感化。
