People's Hospital of Jianyang, Central Laboratory, Jianyang 641400, China.
People's Hospital of Jianyang, Department of Nephrology, Jianyang 641400, China.
Eur Cytokine Netw. 2019 Mar 1;30(1):34-38. doi: 10.1684/ecn.2019.0425.
This study mainly to explore the change of serum cytokines in wasp sting patients and the potential correlation between cytokines and acute kidney injury (AKI) due to wasp stings. The levels of IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ in 33 wasp sting and 24 healthy people were measured by flow cytometry, the level of IL-17 was detected by enzyme-linked immunosorbent assay and the laboratory examination including inflammatory indicators, muscle enzyme markers, and renal function were detected by automatic biochemical analyzer, blood analyzer, and urine analyzer. The wasp sting patients were divided into AKI (n = 10) and non-AKI groups (n = 23). The correlation between the levels of serum cytokines and laboratory examination results was analyzed. The levels of IL-2, IL-6, IL-10, IFN-γ, and IL-17 were statistically increased in wasp sting patients compared with the controls (P < 0.05). IL-6, IL-10, and IL-17 levels were markedly increased in the AKI group compared with the non-AKI group (P < 0.05). Moreover, compared with non-AKI group, inflammatory markers and muscle enzyme markers were more abnormal in the AKI group. The positive rate of urinary occult blood in the AKI group was higher than that in the non-AKI group. The levels of IL-2, IL-4, IL-6, IFN-γ, and IL-17 correlated positively with white blood cell counts. The levels of IL-2, IL-4, IL-10, IFN-γ, and IL-17 correlated positively with the levels of serum creatinine. The levels of IL-2, IL-4, IL-10, IL-10, and IFN-γ correlated positively with the levels of C-reactive protein. The levels of IL-10, and IFN-γ correlated positively with urinary occult blood. Conclusion: Elevated levels of cytokines in wasp sting patients might be involved in the development and progression of acute kidney injury.
本研究主要探讨了蜂蛰伤患者血清细胞因子的变化，以及细胞因子与蜂蛰伤所致急性肾损伤（AKI）之间的潜在相关性。采用流式细胞术检测了 33 例蜂蛰伤患者和 24 例健康人血清中 IL-2、IL-4、IL-6、IL-10、TNF-α和 IFN-γ的水平，酶联免疫吸附试验检测了 IL-17 的水平，并采用自动生化分析仪、血液分析仪和尿液分析仪检测了炎症指标、肌酶标志物和肾功能等实验室检查结果。将蜂蛰伤患者分为 AKI 组（n=10）和非 AKI 组（n=23），分析了血清细胞因子水平与实验室检查结果的相关性。与对照组相比，蜂蛰伤患者血清中 IL-2、IL-6、IL-10、IFN-γ和 IL-17 的水平明显升高（P<0.05）。与非 AKI 组相比，AKI 组的 IL-6、IL-10 和 IL-17 水平明显升高（P<0.05）。此外，与非 AKI 组相比，AKI 组的炎症指标和肌酶标志物更为异常，AKI 组尿潜血的阳性率也高于非 AKI 组。IL-2、IL-4、IL-6、IFN-γ 和 IL-17 与白细胞计数呈正相关，IL-2、IL-4、IL-10、IFN-γ 和 IL-17 与血肌酐水平呈正相关，IL-2、IL-4、IL-10、IL-17 和 IFN-γ 与 C 反应蛋白水平呈正相关，IL-10 和 IFN-γ 与尿潜血呈正相关。结论：蜂蛰伤患者细胞因子水平升高可能参与了急性肾损伤的发生和发展。
