Hoyos-Marulanda V, Alves B S, Rosa P R A, Vieira A D, Gasperin B G, Mondadori R G, Lucia T
ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas, RS, Brazil; Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, 96010-900, Pelotas, RS, Brazil.
ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas, RS, Brazil; Faculdade de Veterinária, Universidade Federal de Pelotas, 96010-900, Pelotas, RS, Brazil.
Anim Reprod Sci. 2019 Jun;205:150-155. doi: 10.1016/j.anireprosci.2019.05.003. Epub 2019 May 6.
As oocytes and embryos of pigs have greater lipid content in the cytoplasm than those of other species, supplementation of the medium for in vitro maturation (IVM) of oocytes with omega-3 polyunsaturated fatty acids (PUFA) may help to improve embryo development. This study was conducted to evaluate effects of the inclusion of the docosaexaenoic (DHA) and of the eicosapentaenoic acids (EPA) in the IVM medium on the development of pig oocytes and on the lipid content of oocytes and embryos. In all experiments, control media consisted of porcine follicular fluid and oocytes were activated through parthenogenesis. In Experiment 1, there were four treatments for each PUFA: one control; and three treatments including EPA or DHA in the IVM medium at 12.5 μM, 25.0 μM and 50.0 μM). In Experiment 2, inclusion of 50 μM DHA was compared against the control. Cleavage rates in the IVM medium including 12.5 μM EPA and blastocyst development rates in media at any EPA concentration were less than for the control in Experiment 1 (P < 0.05). Compared to the control, inclusion of 50 μM DHA in the IVM medium was related to greater cleavage rates and greater number of embryo cells, in Experiment 1, and lesser lipid content in oocytes after 22 and 44 h and in embryos after 7 days, in Experiment 2 (both P < 0.05). Addition of DHA in the IVM medium may benefit the development of pig oocytes, but EPA appears to be cytotoxic.
由于猪的卵母细胞和胚胎细胞质中的脂质含量高于其他物种，在卵母细胞体外成熟（IVM）培养基中添加ω-3多不饱和脂肪酸（PUFA）可能有助于改善胚胎发育。本研究旨在评估在IVM培养基中添加二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）对猪卵母细胞发育以及卵母细胞和胚胎脂质含量的影响。在所有实验中，对照培养基由猪卵泡液组成，卵母细胞通过孤雌生殖激活。在实验1中，每种PUFA有四种处理：一种对照；以及三种处理，即在IVM培养基中分别添加12.5μM、25.0μM和50.0μM的EPA或DHA。在实验2中，将添加50μM DHA与对照进行比较。在实验1中，含12.5μM EPA的IVM培养基中的卵裂率以及任何EPA浓度培养基中的囊胚发育率均低于对照（P < 0.05）。在实验1中，与对照相比，IVM培养基中添加50μM DHA与更高的卵裂率和更多的胚胎细胞数量相关；在实验2中，与对照相比，IVM培养基中添加50μM DHA与22小时和44小时后卵母细胞以及7天后胚胎中较低的脂质含量相关（均P < 0.05）。在IVM培养基中添加DHA可能有利于猪卵母细胞的发育，但EPA似乎具有细胞毒性。
