Department of Animal and Pre-Veterinary Studies, University of Findlay, 1000 North Main Street, Findlay, OH, 45840, USA.
In Vitro Cell Dev Biol Anim. 2022 Aug;58(7):599-609. doi: 10.1007/s11626-022-00708-4. Epub 2022 Aug 4.
Elevated environmental temperatures can induce heat stress which could reduce fertility and early embryonic development. Fatty acids can initiate an endergonic reaction that absorbs cellular heat and decreases intracellular temperature. This study's objective was to minimize heat stress-induced damage to in vitro matured oocytes by supplementing maturation media with either 50 μM linoleic or linolenic acid or both (25 or 50 μM) during maturation at either 38.5 or 41.5°C. Oocytes were evaluated for intracellular antioxidative pathways, fertilization characteristics, or early embryonic development. Elevated maturation temperatures increased (p < 0.05) reactive oxygen species (ROS) formation and supplementation with linoleic or linolenic acid decreased (p < 0.05) ROS in oocytes matured at 41.5°C. Maturation temperature had an effect (p < 0.05) on the intracellular antioxidative pathways of the oocyte except for glutathione peroxidase activity. Regardless of maturation temperature, supplementation with linoleic or linolenic acid increased (p < 0.05) the enzyme activities and glutathione concentrations in the oocytes compared to no fatty acid supplementation. Supplementation of both linoleic and linolenic acid decreased (p < 0.05) polyspermic fertilization rates. Supplementing either 25 or 50 μM linoleic and linolenic acid to maturing oocytes at 41.5°C increased (p < 0.05) cleavage rates by 48 h after IVF and the blastocyst formation rates by 144 h after IVF compared to other treatments. Oocytes matured at 38.5°C had greater (p < 0.05) embryonic development than those matured at 41.5°C except for those supplemented with 50 μM linoleic and linolenic acid. Supplementing 50 μM linoleic and linolenic acid to the maturation medium of pig oocytes reduces the effects of heat stress-induced damage.
升高的环境温度会引起热应激，从而降低生育能力和早期胚胎发育。脂肪酸可以引发吸能反应，吸收细胞热量并降低细胞内温度。本研究的目的是通过在 38.5°C 或 41.5°C 下成熟时在成熟培养基中添加 50 μM 亚油酸或亚麻酸或两者（25 或 50 μM）来最小化热应激对体外成熟卵母细胞的损伤。评估卵母细胞的细胞内抗氧化途径、受精特性或早期胚胎发育。升高的成熟温度增加了（p<0.05）活性氧（ROS）的形成，并且亚油酸或亚麻酸的补充降低了（p<0.05）在 41.5°C 下成熟的卵母细胞中的 ROS。成熟温度对卵母细胞的细胞内抗氧化途径有影响（p<0.05），除了谷胱甘肽过氧化物酶活性外。无论成熟温度如何，与没有脂肪酸补充相比，亚油酸或亚麻酸的补充都会增加（p<0.05）卵母细胞中的酶活性和谷胱甘肽浓度。补充亚油酸和亚麻酸都会降低（p<0.05）多精受精率。在 41.5°C 下向成熟卵母细胞中添加 25 或 50 μM 亚油酸和亚麻酸都会增加（p<0.05）IVF 后 48 小时的卵裂率和 IVF 后 144 小时的囊胚形成率与其他处理相比。在 38.5°C 下成熟的卵母细胞比在 41.5°C 下成熟的卵母细胞具有更高的（p<0.05）胚胎发育能力，除了用 50 μM 亚油酸和亚麻酸补充的卵母细胞。在猪卵母细胞的成熟培养基中添加 50 μM 亚油酸和亚麻酸可以减少热应激诱导的损伤的影响。
