Department of Entomology, University of Minnesota-Twin Cities, Minneapolis, Minnesota, USA
Department of Entomology, University of Minnesota-Twin Cities, Minneapolis, Minnesota, USA.
Appl Environ Microbiol. 2019 Jul 1;85(14). doi: 10.1128/AEM.00866-19. Print 2019 Jul 15.
subsp. is recognized as the etiological agent of human ehrlichiosis in Minnesota and Wisconsin. We describe the culture isolation of this organism from a field-collected tick and detail its relationship to other species of The isolate could be grown in a variety of cultured cell lines and was effectively transmitted between ticks and rodents, with PCR and microscopy demonstrating a broad pattern of dissemination in arthropod and mammalian tissues. Conversely, ticks were not susceptible to infection by the Histologic sections further revealed that the wild-type isolate was highly virulent for mice and hamsters, causing severe systemic disease that was frequently lethal. A transposase system was used to create mCherry- and mKate-expressing EmCRT mutants, which retained the ability to infect rodents and ticks. Ehrlichioses are zoonotic diseases caused by intracellular bacteria that are transmitted by ixodid ticks. Here we report the culture isolation of bacteria which are closely related to, or the same as the subsp. , a recently recognized human pathogen. EmCRT, obtained from a tick removed from deer at Camp Ripley, MN, is the second isolate of this subspecies described and is distinctive in that it was cultured directly from a field-collected tick. The isolate's cellular tropism, pathogenic changes caused in rodent tissues, and tick transmission to and from rodents are detailed in this study. We also describe the genetic mutants created from the EmCRT isolate, which are valuable tools for the further study of this intracellular pathogen.
subsp. 被认为是明尼苏达州和威斯康星州人类埃立克体病的病原体。我们描述了从野外采集的蜱中分离出这种生物体，并详细说明了它与其他 种的关系。该分离株可以在多种培养细胞系中生长，并能有效地在 蜱和啮齿动物之间传播，PCR 和显微镜检查显示在节肢动物和哺乳动物组织中有广泛的传播模式。相反， 蜱对该 株的感染并不敏感。组织学切片进一步表明，野生型分离株对小鼠和仓鼠具有高度致病性，引起严重的全身疾病，常导致死亡。转座酶系统用于创建 mCherry 和 mKate 表达的 EmCRT 突变体，它们保留了感染啮齿动物和蜱的能力。埃立克体病是由节肢动物传播的细胞内细菌引起的人畜共患病。在这里，我们报告了一种与 密切相关或相同的细菌的培养分离，这是一种新确认的人类病原体。EmCRT 是从明尼苏达州里普利营的鹿身上取下的蜱中获得的，是该亚种的第二个分离株，其独特之处在于它是直接从野外采集的蜱中培养出来的。本研究详细描述了该分离株的细胞嗜性、在啮齿动物组织中引起的致病性变化以及在啮齿动物之间的传播。我们还描述了从 EmCRT 分离株中创建的遗传突变体，它们是进一步研究这种细胞内病原体的有用工具。
