Damborský Pavel, Švitel Juraj, Katrlík Jaroslav
Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia.
Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinskeho 9, 81237 Bratislava, Slovakia.
Essays Biochem. 2016 Jun 30;60(1):91-100. doi: 10.1042/EBC20150010.
Optical biosensors represent the most common type of biosensor. Here we provide a brief classification, a description of underlying principles of operation and their bioanalytical applications. The main focus is placed on the most widely used optical biosensors which are surface plasmon resonance (SPR)-based biosensors including SPR imaging and localized SPR. In addition, other optical biosensor systems are described, such as evanescent wave fluorescence and bioluminescent optical fibre biosensors, as well as interferometric, ellipsometric and reflectometric interference spectroscopy and surface-enhanced Raman scattering biosensors. The optical biosensors discussed here allow the sensitive and selective detection of a wide range of analytes including viruses, toxins, drugs, antibodies, tumour biomarkers and tumour cells.
光学生物传感器是最常见的生物传感器类型。在此，我们提供一个简要分类，描述其基本工作原理及其生物分析应用。主要重点放在使用最广泛的光学生物传感器，即基于表面等离子体共振（SPR）的生物传感器，包括SPR成像和局域表面等离子体共振。此外，还描述了其他光学生物传感器系统，如倏逝波荧光和生物发光光纤生物传感器，以及干涉、椭偏和反射干涉光谱和表面增强拉曼散射生物传感器。本文讨论的光学生物传感器能够灵敏且选择性地检测多种分析物，包括病毒、毒素、药物、抗体、肿瘤生物标志物和肿瘤细胞。
