Department of Structural and Functional Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Department of Structural and Functional Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Life Sci. 2019 Jul 15;229:180-186. doi: 10.1016/j.lfs.2019.05.016. Epub 2019 May 8.
The present study aimed to verify changes in cerebellar and plasmatic expression of miRNAs after the chronic consumption of ethanol and caffeine in the UChB rat, an experimental model for alcoholism.
Male rats at 5 months of age, were divided into the following groups (n = 10/group): 1. Ethanol (UChB rats receiving 10% ethanol solution and water ad libitum); 2. Ethanol + caffeine (UChB rats receiving 10% ethanol solution + 3g/l caffeine and water ad libitum); 3. Control (rats receiving water ad libitum). The cerebellum and plasma of the animals were collected and processed by RT-PCR for the miRNAs-155-5p, -146a-5p, -126-3p, -132-3p, -339-5p.
Ethanol and caffeine were capable of regulating the expression of miRNAs associated with the inflammatory process in the tissue and plasma of the UChB rats. Increased expression of the analyzed miRNAs-155-5p, -146a-5p, -126-3p, -132-3p was observed for the cerebellar tissue in the Ethanol group and reduced expression of them in the Ethanol + caffeine group. In plasma, caffeine significantly elevated the miR-126-3p and miR-132-3p levels and decreased miR-155-5p levels. Ethanol consumption increased miR-146a-5p expression and decreased miR-339-5p levels. In brief, altered plasmatic levels of the miRNAs did not reflect the miRNAs levels found in cerebellar tissue.
Considering the results herein, we concluded that ethanol predisposes to an inflammatory process while caffeine has a neuroprotective effect on the cerebellar tissue.
本研究旨在验证慢性摄入乙醇和咖啡因后 UChB 大鼠（酒精中毒的实验模型）小脑和血浆中 miRNA 表达的变化。
5 月龄雄性大鼠分为以下几组（每组 n=10）：1. 乙醇（UChB 大鼠接受 10%乙醇溶液和自由水）；2. 乙醇+咖啡因（UChB 大鼠接受 10%乙醇溶液+3g/l 咖啡因和自由水）；3. 对照组（大鼠自由饮水）。收集动物的小脑和血浆，通过 RT-PCR 处理用于 miRNA-155-5p、-146a-5p、-126-3p、-132-3p、-339-5p 的分析。
乙醇和咖啡因能够调节与 UChB 大鼠组织和血浆中炎症过程相关的 miRNA 表达。在乙醇组中，小脑组织中分析的 miRNA-155-5p、-146a-5p、-126-3p、-132-3p 的表达增加，而在乙醇+咖啡因组中则减少。在血浆中，咖啡因显著升高 miR-126-3p 和 miR-132-3p 水平，降低 miR-155-5p 水平。乙醇消耗增加了 miR-146a-5p 的表达并降低了 miR-339-5p 的水平。简而言之，改变的血浆 miRNA 水平并未反映小脑组织中的 miRNA 水平。
考虑到本文的结果，我们得出结论，乙醇使小脑易发生炎症过程，而咖啡因对小脑组织具有神经保护作用。
