Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou, 510631, PR China.
Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou, 510631, PR China.
Fish Shellfish Immunol. 2019 Jul;90:404-412. doi: 10.1016/j.fsi.2019.05.006. Epub 2019 May 8.
MicroRNAs (miRNAs) are a kind of small non-coding RNAs that have been reported to play a vital role in mediating host-pathogen interactions. High-throughput sequencing technology was applied to identify and illuminate mRNAs and miRNAs from grouper infected with Vibrio alginolyticus. The KEGG pathway enrichment analysis showed that the most significate DEGs are associated with Toll-like receptor signaling pathway and NOD-like receptor signaling pathway. We obtained 374 known miRNAs and 116 novel miRNAs. During them, there are 31 up-regulated miRNAs and 93 down-regulated miRNAs. miRNA-mRNA GO and KEGG analysis show that there are 90 miRNAs associated with the immune system. The target genes of immune-related miRNAs (miR-142, miR-146, miR-150, miR-155, miR-203, miR-205, miR-24, miR-31) and genes (CD80, IL-2, AMPK, PI3K) in Epinephelus coioddes were predicted and validated. This study provides an opportunity to further understanding the molecular mechanisms especially the immune system of miRNA regulation in Epinephelus coioddes host-pathogen interactions.
微小 RNA（miRNAs）是一种小型非编码 RNA，据报道在介导宿主-病原体相互作用方面发挥着重要作用。本研究采用高通量测序技术鉴定并阐明了感染哈维氏弧菌的石斑鱼中的 mRNAs 和 miRNAs。KEGG 通路富集分析显示，最显著的差异表达基因与 Toll 样受体信号通路和 NOD 样受体信号通路有关。我们获得了 374 个已知的 miRNAs 和 116 个新的 miRNAs。其中，有 31 个上调的 miRNAs 和 93 个下调的 miRNAs。miRNA-mRNA GO 和 KEGG 分析显示，有 90 个 miRNAs 与免疫系统有关。预测并验证了与免疫相关的 miRNAs（miR-142、miR-146、miR-150、miR-155、miR-203、miR-205、miR-24、miR-31）及其靶基因（CD80、IL-2、AMPK、PI3K）在卵形鲷中的作用。本研究为进一步了解 miRNA 调控在卵形鲷宿主-病原体相互作用中的分子机制，特别是免疫系统提供了机会。
