Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI, 48824, USA.
Fish Shellfish Immunol. 2019 Aug;91:333-342. doi: 10.1016/j.fsi.2019.05.050. Epub 2019 May 23.
In recent years, microRNAs (miRNAs) have been shown to play important roles in immunity. Analyses of the functions of miRNAs and their targets are useful in understanding the regulation of the immune response. To understand the relationships between miRNAs and their targets during infection, we used zebrafish as an infection model in which to characterize the miRNA and mRNA transcriptomes of zebrafish larvae infected with Vibrio parahaemolyticus. We identified the differentially expressed miRNAs and mRNAs. Overall, 37 known zebrafish miRNAs were differentially expressed in the infection group and 107 predicted target genes of 26 miRNAs were differentially expressed in the mRNA transcriptome. These targets with specific Gene Ontology (GO) terms, such as peripheral nervous system neuron axonogenesis, organophosphate metabolic process, heme binding, protein binding, tetrapyrrole binding, protein dimerization activity, and aromatase activity, which regulate nerve conduction, energy metabolism, hematopoiesis, and protein synthesis. They were also associated with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways such as phototransduction, tryptophan metabolism, notch signaling, and purine metabolism. Our findings indicate that miRNAs regulate the innate immune response via complex networks, and zebrafish (Danio rerio, dre)-miR-205-3p, dre-miR-141-5p, dre-miR-200a-5p, dre-miR-92a-2-5p, dre-miR-192, and dre-miR-1788 may play important roles in the innate immune response by regulating target genes.
近年来，microRNAs（miRNAs）在免疫中发挥着重要作用。分析 miRNAs 和它们的靶基因的功能有助于理解免疫反应的调控。为了了解感染过程中 miRNAs 和它们的靶基因之间的关系，我们使用斑马鱼作为感染模型，来描述感染副溶血弧菌的斑马鱼幼虫的 miRNA 和 mRNA 转录组。我们鉴定了差异表达的 miRNAs 和 mRNAs。总的来说，在感染组中，37 个已知的斑马鱼 miRNAs 发生了差异表达，而 26 个 miRNAs 的 107 个预测靶基因在 mRNA 转录组中发生了差异表达。这些靶基因具有特定的基因本体论（GO）术语，如外周神经系统神经元轴突发生、有机磷代谢过程、血红素结合、蛋白质结合、四吡咯结合、蛋白质二聚化活性和芳香酶活性，这些术语调节神经传导、能量代谢、造血和蛋白质合成。它们还与京都基因与基因组百科全书（KEGG）途径相关，如光转导、色氨酸代谢、Notch 信号和嘌呤代谢。我们的研究结果表明，miRNAs 通过复杂的网络调节先天免疫反应，斑马鱼（Danio rerio，dre）-miR-205-3p、dre-miR-141-5p、dre-miR-200a-5p、dre-miR-92a-2-5p、dre-miR-192 和 dre-miR-1788 可能通过调控靶基因在先天免疫反应中发挥重要作用。
