Lima Verônica Pierzchalski, Tobin Gabriela Cabanas, de Jesus Pereira Mayra Ramos, Silveira Maiele Dornelles, Witz Maria Inês, Nardi Nance Beyer
Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, Avenida Farroupilha 8001, 92425-900 Canoas, RS, Brazil.
Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, Avenida Farroupilha 8001, 92425-900 Canoas, RS, Brazil; CellMed Medicina Regenerativa, Rua da Varzea 22, 91040-600 Porto Alegre, RS, Brazil.
Res Vet Sci. 2019 Jun;124:393-398. doi: 10.1016/j.rvsc.2019.04.022. Epub 2019 Apr 30.
Osteoarthritis associated with hip dysplasia is one of the most common orthopedic abnormalities in dogs, with an incidence of up to 40% in some breeds. Tissue therapy of cartilage has received great attention, with use of mesenchymal stromal cells and different types of biomaterials. The present study aimed to evaluate the effect of platelet lysate (PL) on the proliferation and differentiation of canine adipose tissue-derived mesenchymal stromal cells (ASCs), in liquid culture or hydrogels. PL was prepared from blood collected from healthy dogs and submitted to freezing-thawing cycles, and hydrogel was formed with canine thrombin. The effect of PL on the proliferation and differentiation of canine ASCs was evaluated in liquid and hydrogel systems, with microscopy, quantification of dsDNA, histology and quantification of glycosaminoglycans. The addition of 5% or 10% PL to the culture medium induced a greater proliferation rate than the presence of 10% fetal bovine serum. The cultivation of ASCs in PL gel, with normal or chondrogenic medium, resulted in maintenance of proliferation level similar to the conventional 2D cultivation, and induction of chondrogenic differentiation, especially in the presence of the chondrogenesis induction medium.
与髋关节发育不良相关的骨关节炎是犬类最常见的骨科异常之一，在某些品种中的发病率高达40%。软骨组织治疗受到了广泛关注，包括使用间充质基质细胞和不同类型的生物材料。本研究旨在评估血小板裂解液（PL）在液体培养或水凝胶中对犬脂肪组织来源的间充质基质细胞（ASC）增殖和分化的影响。PL由从健康犬采集的血液制备，并经过冻融循环处理，水凝胶由犬凝血酶形成。通过显微镜检查、双链DNA定量、组织学和糖胺聚糖定量，评估了PL在液体和水凝胶系统中对犬ASC增殖和分化的影响。向培养基中添加5%或10%的PL比添加10%胎牛血清诱导出更高的增殖率。在正常或软骨形成培养基中，将ASC培养在PL凝胶中，其增殖水平维持与传统二维培养相似，并诱导软骨形成分化，特别是在存在软骨形成诱导培养基的情况下。
