Streli C, Rauwolf M, Turyanskaya A, Ingerle D, Wobrauschek P
Atominstitut, TU Wien, Stadionallee 2, 1020, Vienna, Austria.
Atominstitut, TU Wien, Stadionallee 2, 1020, Vienna, Austria.
Appl Radiat Isot. 2019 Jul;149:200-205. doi: 10.1016/j.apradiso.2019.04.033. Epub 2019 Apr 30.
Our group employs micro- and nano-X-ray fluorescence spectrometry (XRF) for the investigation of bone tissue. The manuscript presents the results of 3 various projects, in which spatial distribution of trace elements in bone samples was studied. The first study investigated the distribution of the elemental constituents of Mg-based implants at various stages of the degradation process in surrounding bone tissue with a focus on magnesium (Mg) and yttrium (Y). The analysis was performed in laboratory of Atominstitut using a special micro-XRF spectrometer for light element detection. The second study is devoted to the spatial distribution of Zn in high-grade sclerosing osteosarcoma mapped by confocal synchrotron radiation induced micro-XRF. Tumor zinc levels were compared with adjacent normal tissue. For discrimination between healthy and diseased bone quantitative backscattered electron imaging was used. The third experiment demonstrates the performance of synchrotron radiation induced nano-XRF with beam size of about 500 nm for bone tissue investigation. Special emphasis is set to advantages of micro- and nano-XRF in bone analysis as well as overcoming possible limitations.
我们的团队采用微纳X射线荧光光谱法（XRF）来研究骨组织。该手稿展示了3个不同项目的结果，其中对骨样本中微量元素的空间分布进行了研究。第一项研究调查了镁基植入物在周围骨组织降解过程不同阶段的元素组成分布，重点关注镁（Mg）和钇（Y）。分析是在Atominstitut实验室使用一台用于轻元素检测的特殊微XRF光谱仪进行的。第二项研究致力于通过共聚焦同步辐射诱导微XRF绘制高级别硬化性骨肉瘤中锌的空间分布。将肿瘤锌水平与相邻正常组织进行比较。为了区分健康骨骼和患病骨骼，使用了定量背散射电子成像。第三个实验展示了光束尺寸约为500纳米的同步辐射诱导纳米XRF在骨组织研究中的性能。特别强调了微XRF和纳米XRF在骨分析中的优势以及克服可能的局限性。
