State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, PR China.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
Waste Manag. 2019 Apr 1;88:309-318. doi: 10.1016/j.wasman.2019.03.060. Epub 2019 Mar 30.
Three semi-continuous anaerobic digesters with raw sludge (R1) or thermally hydrolyzed sludge (120 °C (R2) or 160 °C (R3)) were operated to investigate the effects of thermal hydrolysis on the metabolism of amino acids during high solid anaerobic digestion with sewage sludge. Thermal hydrolysis pretreatment (THP) decomposed protein in terms of hydrolytic amino acid in raw sludge by 8.90% and 26.69% under 120 °C and 160 °C, respectively. The decomposition of amino acids during THP was the main contributor to the final enhanced amino acids degradation in sewage sludge with THP after anaerobic digestion. The dominating bacterial genera related to amino acids degradation in R2 and R3 shifted to Fastidiosipila and Proteiniphilum/Tissierella, respectively, from Gelria in R1, influencing the utilization of amino acids, especially glutamic acid, glycine and proline. Different from 120 °C, THP at 160 °C played an important role in promoting amino acid metabolism during AD through the Stickland pathway by the bacteria belonged to order Clostridiales.
三个半连续厌氧消化器分别采用未经热解的污泥（R1）或经 120°C（R2）或 160°C（R3）热解的污泥，研究了热解预处理对高固体厌氧消化中污泥氨基酸代谢的影响。热解预处理（THP）分别使 120°C 和 160°C 下原污泥中的蛋白质以水解氨基酸的形式分解了 8.90%和 26.69%。THP 过程中氨基酸的分解是 THP 后厌氧消化中污泥中最终增强氨基酸降解的主要原因。与 R1 中的 Gelria 相比，R2 和 R3 中与氨基酸降解相关的优势细菌属分别变为 Fastidiosipila 和 Proteiniphilum/Tissierella，影响了氨基酸的利用，特别是谷氨酸、甘氨酸和脯氨酸。与 120°C 不同，160°C 的 THP 通过属于梭菌目（Clostridiales）的细菌通过 Stickland 途径在 AD 过程中促进氨基酸代谢中发挥了重要作用。
