State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, PR China.
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, PR China.
J Hazard Mater. 2018 Jan 15;342:1-9. doi: 10.1016/j.jhazmat.2017.08.012. Epub 2017 Aug 8.
Two lab-scale high solid anaerobic digesters fed with untreated sludge (R1) and thermally hydrolyzed sludge (R2) were operated to investigate the influence of thermal hydrolysis pretreatment (THP) on the degradation of individual macromolecular organic components (MOCs), as well as the functional and metabolic responses of microbes during anaerobic digestion (AD). The degradation of MOCs was improved by THP at different rates, in which improved degradation of proteins (by 49.0%) and hemicelluloses (by 25.0%) were the main factors contributing to the increase in volatile solids (VS) reduction. However, no enhancement of final degradation extent of MOCs was observed. With a more densified microbial population, R2 was also enriched in genes involved in amino acid and carbohydrate metabolism, reflected in the enhanced degradation of proteins and carbohydrates. After THP, the methanogenic pathway shifted from strict acetoclastic methanogenesis to acetoclastic/hydrogenotrophic methanogenesis, consistent with the enhanced methane production and the increase of methane content.
两个采用未经处理的污泥（R1）和热解污泥（R2）的实验室规模高固体厌氧消化器进行了运行，以研究热解预处理（THP）对单个大分子有机成分（MOC）降解的影响，以及在厌氧消化（AD）过程中微生物的功能和代谢反应。THP 以不同的速率提高了 MOC 的降解，其中蛋白质（增加 49.0%）和半纤维素（增加 25.0%）的降解改善是挥发性固体（VS）减少增加的主要因素。然而，并没有观察到 MOC 最终降解程度的提高。由于微生物种群更加密集，R2 还富集了参与氨基酸和碳水化合物代谢的基因，这反映在蛋白质和碳水化合物的降解增强上。经过 THP 后，产甲烷途径从严格的乙酰型产甲烷作用转变为乙酰型/氢营养型产甲烷作用，与甲烷产量的增加和甲烷含量的增加一致。
