Ciaravino V, Meltz M L, Erwin D N
Environ Mutagen. 1987;9(4):393-9. doi: 10.1002/em.2860090405.
Chinese hamster ovary (CHO) cells were exposed for 2 hr with and without mitomycin C (MMC) (1 X 10(-8)M) to pulsed wave radiofrequency radiation (RFR) at 2450 MHz. The repetition rate of 25,000 pulses per sec (pps), pulse width of 10 microseconds, and exposure geometry used, resulted in a specific absorption rate (SAR) of 33.8 W/kg. The following exposure regimens were used: a 37 degrees C water bath control; a water bath temperature control (TC) in which the continuously monitored medium temperature closely followed the temperature rise in the RFR-exposed flasks; and the RFR-exposed cells in a water bath set at 37 degrees C prior to exposure. RFR exposure resulted in a maximum cell culture medium temperature of 39.2 degrees C. In the absence of MMC, there was no significant increase in sister chromatid exchange (SCE) in the RFR-exposed or TC groups over that of the 37 degrees C control. When a simultaneous treatment of RFR and MMC occurred there was no statistical difference in SCE frequency from that caused by chemical treatment alone.
将中国仓鼠卵巢（CHO）细胞在有和没有丝裂霉素C（MMC）（1×10⁻⁸M）的情况下，暴露于2450MHz的脉冲波射频辐射（RFR）中2小时。每秒25000个脉冲（pps）的重复率、10微秒的脉冲宽度以及所使用的暴露几何结构，导致比吸收率（SAR）为33.8W/kg。使用了以下暴露方案：37℃水浴对照组；水浴温度控制组（TC），其中持续监测的培养基温度紧密跟随暴露于RFR的烧瓶中的温度升高；以及在暴露前设置为37℃的水浴中暴露于RFR的细胞。RFR暴露导致细胞培养基最高温度达到39.2℃。在没有MMC的情况下，暴露于RFR或TC组中的姐妹染色单体交换（SCE）与37℃对照组相比没有显著增加。当同时进行RFR和MMC处理时，SCE频率与仅由化学处理引起的频率没有统计学差异。
