Ray M
Cytobios. 1986;45(181):77-84.
Sister chromatid exchanges (SCEs) were investigated in chromosomes from normal male Chinese hamster (CH) and its cell lines (CHW, 1102 and 1103). The fibroblasts were grown for two replication cycles in medium containing BrdU and mitomycin C (MMC) at concentrations of 0.01, 0.02 and 0.03 micrograms/ml of medium. The difference in SCEs/cell between male CH and CHW was negligible, but the difference between CHW and 1102 was about 2.6-fold. It is suggested from karyotypic differences between CHW and 1102, that the control of SCEs might be due partly or completely to chromosome 5 in Chinese hamster. The lines CHW and 1102 were less responsive than normal Chinese hamster cells when exposed to different MMC concentrations. It is suggested that the lines CHW and 1102 might be slightly resistant to MMC. The frequency of SCEs decreased with the decrease of chromosome size. SCEs are not preferentially distributed on any autosomal chromosomes. No SCEs were found in normal X-chromosomes. The majority of exchanges appear to be either interband regions or very near band-interband junctions.
对正常雄性中国仓鼠（CH）及其细胞系（CHW、1102和1103）的染色体进行了姐妹染色单体交换（SCE）研究。成纤维细胞在含有浓度为0.01、0.02和0.03微克/毫升培养基的溴脱氧尿苷（BrdU）和丝裂霉素C（MMC）的培养基中生长两个复制周期。雄性CH和CHW之间每个细胞的SCE差异可忽略不计，但CHW和1102之间的差异约为2.6倍。从CHW和1102之间的核型差异来看，提示中国仓鼠中SCE的控制可能部分或完全归因于5号染色体。当暴露于不同浓度的MMC时，CHW和1102细胞系比正常中国仓鼠细胞反应性更低。提示CHW和1102细胞系可能对MMC有轻微抗性。SCE的频率随染色体大小的减小而降低。SCE并非优先分布于任何常染色体上。在正常X染色体上未发现SCE。大多数交换似乎发生在带间区域或非常靠近带 - 带间交界处。
