Jorgensen K V, Clayton J W, Price R L
Environ Mutagen. 1987;9(4):411-9. doi: 10.1002/em.2860090407.
The effects of glutathione (GSH) and the combination of GSH and glutathione-S-transferase (GST) on aflatoxin B1 (AFB1) mutagenesis in the Salmonella mutagenicity assay using Salmonella typhimurium strains TA98 and TA100 were tested. Ten concentrations of AFB1 (0-1.0 micrograms/plate) were added to a liver microsomal homogenate (S9 mix) or to S9 mix containing GSH or S9 mix containing the combination of GSH + GST. One-third of the samples were plated directly. Two-thirds were incubated for 30 min at 37 degrees C prior to plating, and of those, half included bacteria. All samples were plated according to Ames et al [1975]. The results show that the addition of GSH and GSH + GST affected AFB1 mutagenesis by forming the AFB1-GSH conjugate and decreasing the availability of AFB1-8,9-epoxide. The effect of GST on GSH activity varied with the strain because of the different amounts of S9 mix used. The formation of the AFB1-GSH conjugate was verified by using reverse-phase high-performance liquid chromatography for quantitation of AFB1 and detection of AFB1-GSH.
利用鼠伤寒沙门氏菌TA98和TA100菌株，在沙门氏菌致突变性试验中测试了谷胱甘肽（GSH）以及GSH与谷胱甘肽 - S - 转移酶（GST）的组合对黄曲霉毒素B1（AFB1）诱变作用的影响。将十种浓度的AFB1（0 - 1.0微克/平板）添加到肝微粒体匀浆（S9混合物）中，或添加到含有GSH的S9混合物中，或添加到含有GSH + GST组合的S9混合物中。三分之一的样品直接铺板。三分之二的样品在铺板前于37℃孵育30分钟，其中一半含有细菌。所有样品均按照Ames等人[1975年]的方法铺板。结果表明，添加GSH和GSH + GST通过形成AFB1 - GSH共轭物并降低AFB1 - 8,9 - 环氧化物的可用性来影响AFB1诱变作用。由于使用的S9混合物量不同，GST对GSH活性的影响因菌株而异。通过使用反相高效液相色谱法定量AFB1并检测AFB1 - GSH，验证了AFB1 - GSH共轭物的形成。
