Marques Camila Balconi, Ramos Fernanda, Fernandes Fagner, Sangioni Luís Antônio, Kommers Gláucia, Unterleider Carlos Eduardo, Flores Vogel Fernanda Silveira
Laboratório de Doenças Parasitárias (Ladopar), Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria (UFSM), Av. Roraima 1000, prédio 44, sala 5139, 97105-900 Santa Maria, RS, Brazil.
Laboratório de Doenças Parasitárias (Ladopar), Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria (UFSM), Av. Roraima 1000, prédio 44, sala 5139, 97105-900 Santa Maria, RS, Brazil.
Vet Parasitol. 2019 May;269:53-56. doi: 10.1016/j.vetpar.2019.04.013. Epub 2019 Apr 29.
Infestations of Rhipicephalus microplus cause significant damage to cattle breeding and their control is primarily based on chemical products. There are extensive reports of efficacy losses of acaricid products over time, as well as resistance of the parasites to them, thereby making it necessary to search for new alternatives. The present work aimed to determine the in vitro and in vivo acaricidal activity of organo-modified siloxanes with and without piperonyl butoxide (PBO) in southern Brazil. For the in vitro test, engorged females of R. microplus were collected and submitted to the immersion test. The formulation containing organo-modified siloxanes was tested at 4 different concentrations: 0.6, 1.0, 2.5, and 5.0%. The lowest dilutions (0.6, 1.0, and 2.5%) were also tested with the inclusion of 10% PBO. The in vivo test was performed by applying 2.5% organo-modified siloxanes, and the addition of 10% PBO was applied by spraying. The results showed high acaricidal activity (100%) in vitro in the concentration of 5% on non-associated forms, and in combinations of concentrations of 0.6, 1.0, 2.5% with PBO. The in vivo results also increased the efficacy with the association of PBO. In view of the current multi-resistance scenario of the R. microplus tick to the different commercially available acaricidal products, this study investigated the use of this product in association with PBO as an alternative to R. microplus control and found positive results. To the authors' knowledge, this is the first study to use organo-modified siloxanes against ticks.
微小扇头蜱的侵扰对养牛业造成了重大损害，其防治主要基于化学产品。随着时间的推移，杀螨剂产品效力损失以及寄生虫对其产生抗性的报道广泛存在，因此有必要寻找新的替代方法。本研究旨在确定在巴西南部，含与不含胡椒基丁醚（PBO）的有机改性硅氧烷的体外和体内杀螨活性。对于体外试验，收集饱血的微小扇头蜱雌蜱并进行浸渍试验。含有机改性硅氧烷的制剂在4种不同浓度下进行测试：0.6%、1.0%、2.5%和5.0%。最低稀释度（0.6%、1.0%和2.5%）还添加10%的PBO进行测试。体内试验通过涂抹2.5%的有机改性硅氧烷进行，添加10%的PBO则通过喷雾进行。结果表明，在体外，5%浓度对非关联形态具有高杀螨活性（100%），0.6%、1.0%、2.5%浓度与PBO组合时也具有高杀螨活性。体内试验结果也表明PBO组合提高了效力。鉴于目前微小扇头蜱对不同市售杀螨产品的多重抗性情况，本研究调查了该产品与PBO联合使用作为微小扇头蜱防治替代方法的效果，并取得了积极结果。据作者所知，这是第一项使用有机改性硅氧烷防治蜱虫的研究。
