Jaiswal Amit Kumar, Choudhury Soumen, Agrawal Vivek, Singh Amit, Kumar Muneendra
Department of Veterinary Parasitology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, Uttar Pradesh, India.
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, Uttar Pradesh, India.
Trop Anim Health Prod. 2025 May 31;57(5):239. doi: 10.1007/s11250-025-04492-7.
This study investigated the acaricidal efficacy of eugenol and 1,8-cineole against Rhipicephalus (Boophilus) microplus ticks using in vitro adult immersion test (AIT) and larval packet test (LPT). Eugenol and 1,8-cineole were tested at concentrations of 80, 40, 20, 10, and 5 mg/mL for AIT. The concentrations of eugenol used were 100, 50, 25, 12.2, 6.25, 3.125, 1.56, and 0.781 mg/mL and 1, 8 cineole were 100, 50, 25, 12.2, 6.25, 3.125 mg/mL for LPT. The results showed that eugenol achieved 100% mortality for adult ticks at 80 mg/mL and for larvae at 6.25 mg/mL, indicated a high level of efficacy. In contrast, 1,8-cineole resulted in 60% adult tick mortality at 80 mg/mL and 80% larval mortality at 100 mg/mL. The LC values were determined as 4.20 mg/mL and 1.808 mg/mL for eugenol in AIT and LPT, respectively, while 1,8-cineole had LC values of 5.70 mg/mL and 16.34 mg/mL for AIT and LPT, respectively. These findings indicated that eugenol is more effective than 1,8-cineole in killing tick larvae, although both compounds demonstrated similar efficacy in adult tick death by LC. The better efficacy of eugenol suggested its potential as potent herbal acaricide, while 1,8-cineole still shows promise as an alternative. The results supported the potential of these compounds for developing effective, herbal-based tick control solutions.
本研究采用体外成虫浸泡试验（AIT）和幼虫包囊试验（LPT），调查了丁香酚和1,8-桉叶素对微小牛蜱的杀螨效果。在AIT中，丁香酚和1,8-桉叶素的测试浓度分别为80、40、20、10和5毫克/毫升。LPT中使用的丁香酚浓度为100、50、25、12.2、6.25、3.125、1.56和0.781毫克/毫升，1,8-桉叶素的浓度为100、50、25、12.2、6.25、3.125毫克/毫升。结果表明，丁香酚在80毫克/毫升时对成虫蜱的死亡率达到100%，在6.25毫克/毫升时对幼虫的死亡率达到100%，显示出较高的药效。相比之下，1,8-桉叶素在80毫克/毫升时导致成虫蜱死亡率为60%，在100毫克/毫升时导致幼虫死亡率为80%。在AIT和LPT中，丁香酚的LC值分别测定为4.20毫克/毫升和1.808毫克/毫升，而1,8-桉叶素在AIT和LPT中的LC值分别为5.70毫克/毫升和16.34毫克/毫升。这些发现表明，尽管两种化合物通过LC在杀死成虫蜱方面表现出相似的效果，但丁香酚在杀死蜱幼虫方面比1,8-桉叶素更有效。丁香酚更好的药效表明其作为强效草药杀螨剂的潜力，而1,8-桉叶素仍有望作为替代品。结果支持了这些化合物开发有效、基于草药的蜱虫控制解决方案的潜力。
