Abazari Mohammad Foad, Soleimanifar Fatemeh, Enderami Seyed Ehsan, Nematzadeh Mahsa, Nasiri Navid, Nejati Fatemeh, Saburi Ehsan, Khodashenas Shabanali, Darbasizadeh Behzad, Khani Mohammad Mehdi, Ghoraeian Pegah
Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran.
Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran.
J Cell Biochem. 2019 Oct;120(10):16750-16759. doi: 10.1002/jcb.28933. Epub 2019 May 13.
Bioactive scaffolds that can increase transplanted cell survival time at the defect site have a great promising potential to use clinically since tissue regeneration or secretions crucially depend on the transplanted cell survival. In this study embedded basic fibroblast growth factor (bFGF)-polycaprolactone-polyvinylidene fluoride (PCL-PVDF) hybrid was designed and fabricated by electrospinning as a bio-functional nanofibrous scaffold for bone tissue engineering. After morphological characterization of the PCL-PVDF (bFGF) scaffold, nanofibers biocompatibility was investigated by culturing of the human induced pluripotent stem cells (iPSCs). Then, the bone differentiation capacity of the iPSCs was evaluated when grown on the PCL-PVDF and PCL-PVDF (bFGF) scaffolds in comparison with culture plate as a control using evaluating of the common osteogenic markers. The viability assay displayed a significant increase in iPSCs survival rate when grown on the bFGF content scaffold. The highest alkaline phosphatase activity and mineralization were detected in the iPSCs while grown on the PCL-PVDF (bFGF) scaffolds. Obtained results from gene and protein expression were also demonstrated the higher osteoinductive property of the bFGF content scaffold compared with the scaffold without it. According to the results, the release of bFGF from PCL-PVDF nanofibers increased survival and proliferation rate of the iPSCs, which followed by an increase in its osteogenic differentiation potential. Taking together, PCL-PVDF (bFGF) nanofibrous scaffold demonstrated that can be noted as a promising candidate for treating the bone lesions by tissue engineering products.
由于组织再生或分泌物严重依赖于移植细胞的存活，能够延长移植细胞在缺损部位存活时间的生物活性支架在临床应用中具有巨大的潜力。在本研究中，通过静电纺丝设计并制备了嵌入碱性成纤维细胞生长因子（bFGF）的聚己内酯-聚偏二氟乙烯（PCL-PVDF）复合材料，作为用于骨组织工程的生物功能纳米纤维支架。对PCL-PVDF（bFGF）支架进行形态表征后，通过培养人诱导多能干细胞（iPSCs）研究了纳米纤维的生物相容性。然后，与作为对照的培养板相比，在PCL-PVDF和PCL-PVDF（bFGF）支架上培养iPSCs时，通过评估常见的成骨标志物来评价iPSCs的骨分化能力。活力测定显示，在含bFGF的支架上生长时，iPSCs的存活率显著提高。在PCL-PVDF（bFGF）支架上培养的iPSCs中检测到最高的碱性磷酸酶活性和矿化程度。基因和蛋白质表达的结果也表明，含bFGF的支架比不含bFGF的支架具有更高的骨诱导性能。根据结果，bFGF从PCL-PVDF纳米纤维中的释放增加了iPSCs的存活和增殖率，随后其成骨分化潜能也增加。综上所述，PCL-PVDF（bFGF）纳米纤维支架表明可被视为通过组织工程产品治疗骨损伤的有前景的候选材料。
