Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran.
J Cell Biochem. 2019 Apr;120(4):6339-6346. doi: 10.1002/jcb.27921. Epub 2018 Oct 15.
Implants that can enhance the stem cells differentiation in the absence of the chemical osteogenic growth factors will attract the great interest of orthopedic scientists. Inorganic polyphosphate (poly-P), as a ubiquitous biological polymer, is one of the factors that can be an alternative for osteogenic growth factors via activating Wnt/β-catenin signaling. In this study, poly-P was incorporated at the blend of polycaprolactone (PCL)/poly (l-lactic acid) (PLLA) electrospun nanofibers and then osteogenic differentiation potential of human-induced pluripotent stem cells (iPSCs) was investigated by the important bone markers. 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) and scanning electron microscopy results confirmed the biocompatibility of the fabricated nanofibers, while higher proliferation rate of iPSCs was detected in PCL-PLLA(poly-P) group compared with the PCL-PLLA and tissue culture plate groups. Alkaline phosphatase activity, calcium content, and gene expression results demonstrated that osteogenic differentiation of iPSCs was increased when cultured on PCL-PLLA(poly-P) in comparison with other groups. According to the results, PCL-PLLA(poly-P) could be considered as a promising candidate for use as bone implants.
在不存在化学成骨生长因子的情况下能够增强干细胞分化的植入物将吸引骨科科学家的极大兴趣。多聚磷酸（poly-P）作为一种普遍存在的生物聚合物，是通过激活 Wnt/β-连环蛋白信号通路替代成骨生长因子的因素之一。在这项研究中，将多聚磷酸掺入聚己内酯（PCL）/聚（L-乳酸）（PLLA）电纺纳米纤维的混合物中，然后通过重要的骨标志物研究人诱导多能干细胞（iPSCs）的成骨分化潜力。3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）和扫描电子显微镜结果证实了所制备的纳米纤维的生物相容性，而与 PCL-PLLA 和组织培养板组相比，iPSCs 在 PCL-PLLA（poly-P）组中的增殖率更高。碱性磷酸酶活性、钙含量和基因表达结果表明，与其他组相比，iPSCs 在 PCL-PLLA（poly-P）上培养时成骨分化增加。根据这些结果，PCL-PLLA（poly-P）可以被认为是用作骨植入物的有前途的候选物。
