Stern Norman J, Line J Eric
Richard B. Russell Agricultural Research Center, Poultry Microbiological Safety Research Unit, U. S. Department of Agriculture, P. O. Box 5677 Athens, Georgia 30613.
Department of Food Science, University of Georgia, Griffin, Georgia 30223.
J Food Prot. 1992 Sep;55(9):663-666. doi: 10.4315/0362-028X-55.9.663.
We compared three enrichment methods, with different sampling times (mid-and endpoint incubation) and various dilutions of enrichment culture for productivity in the isolation of Campylobacter spp. from 50 retail-level chicken carcasses. We subcultured the enrichment cultures onto three Campylobacter spp.-selective media (Campy-BAP, CCDA, Campy-Cefex) to compare yields of the organism. The highest yield (43/50) of Campylobacter spp. from these carcasses was derived by using the 24-h enrichment culture of Hunt & Radle diluted 1:100 before plating onto any of the three selective plating media. When all carcass analyses were combined, Campylobacter spp. was recovered from 49 of 50 broilers. This study indicates the optimum approaches for the recovery of Campylobacter spp., as well as the high incidence of the organism among the broiler carcasses tested.
我们比较了三种富集方法，采用不同的采样时间（培养中期和终点）以及富集培养物的各种稀释度，以评估从50份零售级鸡 carcasses 中分离弯曲杆菌属的效率。我们将富集培养物转接至三种弯曲杆菌属选择性培养基（Campy-BAP、CCDA、Campy-Cefex）上，以比较该菌的产量。这些 carcasses 中弯曲杆菌属的最高产量（43/50）是通过使用Hunt & Radle的24小时富集培养物，在接种到任何一种三种选择性平板培养基之前稀释1:100获得的。当所有 carcass 分析合并时，从50只肉鸡中的49只中检出了弯曲杆菌属。本研究表明了弯曲杆菌属的最佳回收方法，以及在所测试的肉鸡 carcasses 中该菌的高发生率。
