Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
Nanomedicine (Lond). 2019 May;14(10):1247-1265. doi: 10.2217/nnm-2018-0405. Epub 2019 May 14.
Plant virus-like particles (VLPs) have emerged as a novel platform for delivery of drugs/antibodies. The aim of the present investigation is to establish the entry mechanism of flexuous rod-shaped virus particles into mammalian cells. Far-Western blot analysis, pull-down and ELISA were used to characterize vimentin and Hsp60 interaction with VLPs. The mode/kinetics of internalization of VLPs was deciphered using pharmacological inhibitors/endosomal markers. The flexuous rod-shaped VLPs of Pepper vein banding virus (PVBV) enter HeLa and HepG2 cells via cell-surface proteins: vimentin and Hsp60, respectively. VLPs internalize via different modes of endocytosis in HeLa, HepG2 cells and are biodegradable. Vimentin and Hsp60 could be potential epithelial ligands that facilitate targeting of nanoparticles to tumor cells.
植物病毒样颗粒 (VLPs) 已成为一种将药物/抗体递送至细胞内的新型平台。本研究旨在建立弯曲杆状病毒颗粒进入哺乳动物细胞的进入机制。利用 Far-Western blot 分析、下拉实验和 ELISA 来鉴定中间丝蛋白和热休克蛋白与 VLP 的相互作用。利用药理学抑制剂/内体标记物来解析 VLP 的内化模式/动力学。辣椒叶脉坏死病毒（PVBV）的弯曲杆状 VLPs 通过细胞表面蛋白：波形蛋白和热休克蛋白 60 分别进入 HeLa 和 HepG2 细胞。VLPs 通过不同的内吞作用方式内化，并可生物降解。波形蛋白和热休克蛋白 60 可能是上皮细胞的潜在配体，有助于将纳米颗粒靶向肿瘤细胞。
