a Laboratorio de Farmacología , Centro de Investigación Veterinaria de Tandil (CIVETAN) , Tandil , Argentina.
b Facultad de Ciencias Veterinarias , UNCPBA-CICPBA-CONICET , Tandil , Argentina.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2019 Jul;36(7):1055-1067. doi: 10.1080/19440049.2019.1609704. Epub 2019 May 14.
The available information on drug residue stability in chicken egg is scarce. The objective of this study was to evaluate the stability of drug residues in egg under different traditional cooking procedures. Fresh eggs were spiked with different drug concentrations of albendazole (ABZ) and its albendazole sulphoxide (ABZSO) and albendazole sulphone (ABZSO) metabolites; flubendazole (FLBZ) and its reduced flubendazole (R-FLBZ) and hydrolyzed flubendazole (H-FLBZ) metabolites; amoxicillin (AMX); and enrofloxacin (EFX) and its ciprofloxacin (CFX) metabolite. The egg samples were cooked in different ways, namely, boiling, microwaving, and omelette making. Drug residue concentrations in egg were quantified by HPLC with UV or fluorescence detectors. ABZ and ABZSO concentrations in egg were not affected by boiling and microwaving, while the omelette processing significantly reduced these molecules. Residues of ABZSO in egg were stable or increased after all cooking procedures. In contrast, FLBZ and its metabolites FLBZ-H and FLBZ-R residues in egg decreased after all treatments. The residue concentration quantified for EFX and CFX did not show significant changes after any cooking method. AMX residues were unstable, with extremely significant drug reduction after all cooking processes. Conventional methods of egg cooking cannot be considered a tool to eliminate all veterinary drug residues.
有关鸡卵中药物残留稳定性的可用信息很少。本研究的目的是评估不同传统烹饪程序下卵中药物残留的稳定性。新鲜鸡蛋中添加了不同浓度的阿苯达唑（ABZ）及其阿苯达唑砜（ABZSO）和阿苯达唑砜（ABZSO）代谢物；氟苯达唑（FLBZ）及其还原氟苯达唑（R-FLBZ）和水解氟苯达唑（H-FLBZ）代谢物；阿莫西林（AMX）；恩诺沙星（EFX）及其环丙沙星（CFX）代谢物。鸡蛋样品采用不同方法烹饪，即煮沸、微波和煎蛋。采用 HPLC 结合紫外或荧光检测器定量测定鸡蛋中的药物残留浓度。ABZ 和 ABZSO 浓度在鸡蛋中不受煮沸和微波的影响，而煎蛋加工则显著降低了这些分子。在所有烹饪过程后，ABZSO 在鸡蛋中的残留稳定或增加。相比之下，FLBZ 及其代谢物 FLBZ-H 和 FLBZ-R 在鸡蛋中的残留量在所有处理后均减少。经任何烹饪方法处理后，EFX 和 CFX 的残留浓度均未显示出明显变化。AMX 残留不稳定，所有烹饪过程后药物残留量均显著降低。传统的鸡蛋烹饪方法不能被视为消除所有兽药残留的工具。
