Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute; and the Zernike Institute for Advanced Materials, University of Groningen, 9747 AG Groningen, Netherlands; email:
Current affiliation: Biophysics Group, AMOLF, 1098 XG Amsterdam, Netherlands.
Annu Rev Biophys. 2019 May 6;48:185-207. doi: 10.1146/annurev-biophys-052118-115352.
Single-molecule studies provide unprecedented details about processes that are difficult to grasp by bulk biochemical assays that yield ensemble-averaged results. One of these processes is the translocation and insertion of proteins across and into the bacterial cytoplasmic membrane. This process is facilitated by the universally conserved secretion (Sec) system, a multi-subunit membrane protein complex that consists of dissociable cytoplasmic targeting components, a molecular motor, a protein-conducting membrane pore, and accessory membrane proteins. Here, we review recent insights into the mechanisms of protein translocation and membrane protein insertion from single-molecule studies.
单分子研究提供了前所未有的细节，这些细节很难通过产生平均结果的批量生化分析来理解。其中一个过程是蛋白质跨膜和插入细菌细胞质膜的易位和插入。这个过程是由普遍保守的分泌（Sec）系统来促进的，它是一个多亚基膜蛋白复合物，由可分离的细胞质靶向成分、分子马达、蛋白质传导膜孔和辅助膜蛋白组成。在这里，我们回顾了单分子研究中对蛋白质易位和膜蛋白插入机制的最新见解。
