MOE Key Laboratory of Laser Life Science & College of Biophotonics, South China Normal University, Guangzhou, 510631, China.
MOE Key Laboratory of Laser Life Science & College of Biophotonics, South China Normal University, Guangzhou, 510631, China.
Biochem Biophys Res Commun. 2019 Jun 30;514(3):881-887. doi: 10.1016/j.bbrc.2019.05.039. Epub 2019 May 10.
Bax oligomerization is essential for triggering mitochondrial outer membrane permeabilization (MOMP) in many apoptotic programs. However, it is controversial whether Bax dimer is sufficient to trigger MOMP. In this report, multiple Gaussian function-based FRET analysis (Multi-Gaussian FRET analysis) was used to dissect the dimerization and then tetramerization of Bax in relation to MOMP. Multi-Gaussian FRET analysis on the time-lapse FRET images of single living cells co-expressing CFP-Bax and YFP-Bax revealed that formation of mitochondrial Bax homodimers preceded MOMP within 3 min and Bax dimer transformed into tetramer within 6 min concomitantly with complete MOMP within 10 min, providing direct evidence in support of the sufficient ability of Bax dimers to trigger MOMP at least in natural cells.
Bax 寡聚化对于触发许多凋亡程序中的线粒体外膜通透性（MOMP）是必不可少的。然而，Bax 二聚体是否足以触发 MOMP 仍存在争议。在本报告中，使用基于多重高斯函数的 FRET 分析（Multi-Gaussian FRET analysis）来剖析 Bax 的二聚化，然后是四聚化与 MOMP 的关系。对共表达 CFP-Bax 和 YFP-Bax 的单个活细胞的延时 FRET 图像进行多重高斯 FRET 分析表明，线粒体 Bax 同源二聚体的形成先于 MOMP，在 3 分钟内，Bax 二聚体在 6 分钟内转化为四聚体，同时在 10 分钟内完全 MOMP，为 Bax 二聚体至少在天然细胞中足以触发 MOMP 的充分能力提供了直接证据。
