College of Life Science, Northeast Forestry University, Harbin, China.
Exp Cell Res. 2019 Aug 1;381(1):139-149. doi: 10.1016/j.yexcr.2019.05.004. Epub 2019 May 11.
Guanine-rich RNA sequence binding factor 1 (GRSF1) is a member of the RNA-binding protein (RBP) family. GRSF1 regulates RNA metabolism through RNA processing, transport and translation in the cytoplasm and mitochondria. However, its role in myogenesis has not been investigated. Here, we demonstrated that the expression of mitochondrial GRSF1 was negatively related to the differentiation of mouse skeletal myoblasts. Interference with GRSF1 promotes myogenesis both in vitro and in vivo without affecting MyoD expression or cell proliferation. Further studies illustrated that GRSF1 regulated myogenic differentiation through direct targeting of mitochondrial GPX4, a key regulator of the cellular redox status, leading to the modulation of ROS levels, which is important for myogenesis. Our findings underscore a critical function for GRSF1 during skeletal myogenesis linked to its regulation of muscle redox homeostasis.
富含鸟嘌呤的 RNA 序列结合因子 1（GRSF1）是 RNA 结合蛋白（RBP）家族的成员。GRSF1 通过细胞质和线粒体中的 RNA 加工、运输和翻译来调节 RNA 代谢。然而，其在肌发生中的作用尚未被研究。在这里，我们证明线粒体 GRSF1 的表达与小鼠骨骼肌成肌细胞的分化呈负相关。干扰 GRSF1 促进了体外和体内的肌发生，而不影响 MyoD 表达或细胞增殖。进一步的研究表明，GRSF1 通过直接靶向细胞氧化还原状态的关键调节剂线粒体 GPX4 来调节成肌分化，导致 ROS 水平的调节，这对于肌发生很重要。我们的研究结果强调了 GRSF1 在骨骼肌发生过程中的关键作用，与其调节肌肉氧化还原平衡有关。
