Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Republic of Korea.
Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Republic of Korea, Phone: +82-51-999-5908, Fax: +82-51-999-5457.
Z Naturforsch C J Biosci. 2019 Jul 26;74(7-8):167-174. doi: 10.1515/znc-2018-0044.
Osteoporosis is one of the most common bone diseases, occurring due to an imbalance between bone formation and bone resorption. The aim of this study was to investigate the effects of Ishige sinicola, a brown alga, on osteoblast differentiation through the activation of the bone morphogenetic protein 2 (BMP-2)/runt-related transcription factor 2 (Runx2) signalling pathway in MC3T3-E1 cells. A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining, and expression analysis of osteoblastic genes were carried out to assess MC3T3-E1 cell proliferation and osteoblastic differentiation. We found that I. sinicola extract (ISE) increased cell proliferation in a dose-dependent manner. Ishige sinicola extract markedly promoted ALP activity and mineralization. Alizarin red S staining demonstrated that ISE treatment tended to increase extracellular matrix calcium accumulation. Moreover, ISE up-regulated the osteoprotegerin/receptor activator of nuclear factor κB ligand ratio. Ishige sinicola extract also increased the protein expression levels of type 1 collagen, ALP, osteocalcin, osterix, BMP-2, and Runx2. Therefore, ISE showed potential in stimulating osteoblastic bone formation, and it might be useful for the prevention and treatment of osteoporosis.
骨质疏松症是最常见的骨骼疾病之一，其发生是由于骨形成和骨吸收之间的失衡所致。本研究旨在通过激活骨形态发生蛋白 2（BMP-2）/ runt 相关转录因子 2（Runx2）信号通路，研究石花菜（一种褐藻）对成骨细胞分化的影响。通过细胞增殖试验、碱性磷酸酶（ALP）活性测定、茜素红染色和成骨细胞基因表达分析来评估 MC3T3-E1 细胞的增殖和成骨分化。结果发现，石花菜提取物（ISE）呈剂量依赖性地促进细胞增殖。ISE 明显促进 ALP 活性和矿化。茜素红 S 染色表明，ISE 处理可增加细胞外基质钙的积累。此外，ISE 还增加了护骨素/核因子 κB 配体受体激活剂的比值。ISE 还增加了Ⅰ型胶原、ALP、骨钙素、osterix、BMP-2 和 Runx2 的蛋白表达水平。因此，ISE 具有刺激成骨细胞骨形成的潜力，可用于预防和治疗骨质疏松症。
