Department of Food and Nutrition, College of Health and Welfare, Silla University, Busan 46958, Korea.
Nutrients. 2022 Apr 19;14(9):1683. doi: 10.3390/nu14091683.
Osteoporosis is characterized by reduction in bone mass and microarchitectural deterioration of the bone, which causes bone fragility and fracture susceptibility. , a brown alga, reportedly affects osteoblast differentiation. However, its protective effect on estrogen deficiency-induced bone loss has not been elucidated. This study aimed to investigate the effect of extract (ISE) on ovariectomy (OVX)-induced bone loss in vivo and osteoclastogenesis in vitro. Female Sprague-Dawley rats were randomly assigned to the sham-operated (SHAM) group and four OVX subgroups: SHAM, OVX, ISE20 (20 mg/kg), ISE200 (200 mg/kg), and estradiol (10 μg/kg). After 6 weeks of treatment, the bone mineral density (BMD), femur indices, and serum biomarker levels were measured. Furthermore, the effects of ISE on osteoclastogenesis and the expression of osteoclast-specific markers were measured. ISE administration improved the trabecular bone structure, bone biomechanical properties, BMD, and bone mineralization degree. In addition, the levels of serum bone turnover markers were decreased in the ISE group compared with those in the OVX group. Moreover, ISE inhibited osteoclast formation by downregulating NFATc1, TRAP, c-Src, c-Fos, and cathepsin K without any cytotoxic effects on RANKL-induced osteoclast formation. Therefore, we suggest that ISE has therapeutic potential in postmenopausal osteoporosis.
骨质疏松症的特征是骨量减少和骨微观结构恶化，导致骨脆弱和骨折易感性。据报道，一种褐藻能影响成骨细胞分化。然而，其对雌激素缺乏引起的骨丢失的保护作用尚未阐明。本研究旨在探讨 提取物（ISE）对去卵巢（OVX）诱导的体内骨丢失和体外破骨细胞形成的影响。雌性 Sprague-Dawley 大鼠随机分为假手术（SHAM）组和四个 OVX 亚组：SHAM、OVX、ISE20（20mg/kg）、ISE200（200mg/kg）和雌二醇（10μg/kg）。治疗 6 周后，测量骨矿物质密度（BMD）、股骨指数和血清生物标志物水平。此外，还测量了 ISE 对破骨细胞形成和破骨细胞特异性标志物表达的影响。ISE 给药改善了小梁骨结构、骨生物力学特性、BMD 和骨矿化程度。此外，ISE 组血清骨转换标志物水平较 OVX 组降低。此外，ISE 通过下调 NFATc1、TRAP、c-Src、c-Fos 和组织蛋白酶 K 抑制破骨细胞形成，而对 RANKL 诱导的破骨细胞形成没有任何细胞毒性作用。因此，我们认为 ISE 具有治疗绝经后骨质疏松症的潜力。
