可注射磷酸钙骨水泥填充大鼠颅骨关键缺损修复评估
Critical Defect Healing Assessment in Rat Calvaria Filled with Injectable Calcium Phosphate Cement.
作者信息
Schmidt Luis Eduardo, Hadad Henrique, Vasconcelos Igor Rodrigues de, Colombo Luara Teixeira, da Silva Rodrigo Capalbo, Santos Ana Flavia Piquera, Cervantes Lara Cristina Cunha, Poli Pier Paolo, Signorino Fabrizio, Maiorana Carlo, Carvalho Paulo Sérgio Perri de, Souza Francisley Ávila
机构信息
Implant Dentistry Post-Graduation Program, São Leopoldo Mandic School of Dentistry and Research Center, Campinas 13.045-755, Brazil.
Department of Surgery and Integrated Clinic, Araçatuba Dental of School, São Paulo State University Júlio de Mesquita Filho-UNESP, Araçatuba, São Paulo 16.015.050, Brazil.
出版信息
J Funct Biomater. 2019 May 13;10(2):21. doi: 10.3390/jfb10020021.
(1) Background: The tissue engineering field has been working to find biomaterials that mimic the biological properties of autogenous bone grafts. (2) Aim: To evaluate the osteoconduction potential of injectable calcium phosphate cement implanted in critical defects in rat calvaria. (3) Methods: In the calvarial bone of 36 rats, 7-mm diameter critical size defects were performed. Afterwards, the animals were randomly divided into three groups according to filler material: a blood clot group (BC), blood clot membrane group (BCM), and an injectable β-tricalcium phosphate group (HBS) cement group. After periods of 30 and 60 days, the animals were euthanized, the calvaria was isolated, and submitted to a decalcification process for later blades confection. Qualitative and quantitative analysis of the neoformed bone tissue were conducted, and histometric data were statistically analyzed. (4) Results: Sixty days post-surgery, the percentages of neoformed bone were 10.67 ± 5.57 in group BC, 16.71 ± 5.0 in group BCM, and 55.11 ± 13.20 in group HBS. The bone formation values in group HBS were significantly higher ( < 0.05) than in groups BC and BCM. (5) Conclusions: Based on these results, it can be concluded that injectable calcium phosphate cement is an osteoconductive material that can be used to fill bone cavities.
(1) 背景：组织工程领域一直在努力寻找能够模拟自体骨移植生物特性的生物材料。(2) 目的：评估植入大鼠颅骨临界缺损处的可注射磷酸钙骨水泥的骨传导潜力。(3) 方法：在36只大鼠的颅骨上制作直径7毫米的临界尺寸缺损。之后，根据填充材料将动物随机分为三组：血凝块组（BC）、血凝块膜组（BCM）和可注射β-磷酸三钙（HBS）骨水泥组。在30天和60天后，对动物实施安乐死，分离颅骨，并进行脱钙处理以便后续制作切片。对新形成的骨组织进行定性和定量分析，并对组织计量学数据进行统计分析。(4) 结果：术后60天，BC组新形成骨的百分比为10.67±5.57，BCM组为16.71±5.0，HBS组为55.11±13.20。HBS组的骨形成值显著高于（<0.05）BC组和BCM组。(5) 结论：基于这些结果，可以得出结论，可注射磷酸钙骨水泥是一种可用于填充骨腔的骨传导材料。
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