de Oliveira Júlio César Silva, Baggio Ana Maira Pereira, Benetti Luan Pier, Delamura Izabela Fornazari, Ramos Edith Umasi, Bizelli Vinícius Ferreira, Bassi Ana Paula Farnezi
Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, SP, Brazil.
Bioengineering (Basel). 2022 Dec 21;10(1):15. doi: 10.3390/bioengineering10010015.
The membranes are an important biomaterial that contribute to osteopromotion. This study aimed to evaluate the osteopromotive potential of collagen membranes associated with Hydroxyapatite (HA) in critical size calvaria rat's defects. Ninety-six Albinus Wistar rats were divided into four groups: (CG) negative control: clot only (CG); positive control: porcine collagen membrane (BG); fish collagen membrane associated with HA (CP); bovine collagen membrane associated with HA (CB), analyzed at 7, 15, 30, and 60 postoperative days. At 30 days, membrane integrity was observed in the CB and fragments in the CP and BG groups were dispersed in the center of the defect. At 60 days, BG demonstrated better results with no statistical difference for the CP group ( = 0.199) and a statistically significant difference for the CB group ( = 0.013). The inflammatory profiles of the BG and CP groups were similar. Immunohistochemistry demonstrated at 60 days moderate osteopontin staining for the BG and CP groups, light staining for the CB, and intense osteocalcin staining for the BG, while the CB and CP groups demonstrated moderate staining. Microtomography revealed the highest mean bone volume (14.247 mm) in the BG, followed by the CB (11.850 mm), and CP (9.560 mm) group. The collagen membranes associated with HA demonstrated an osteopromotive potential.
这些膜是有助于骨促进的重要生物材料。本研究旨在评估与羟基磷灰石（HA）相关的胶原膜在大鼠临界大小颅骨缺损中的骨促进潜力。将96只白化Wistar大鼠分为四组：（CG）阴性对照组：仅血凝块（CG）；阳性对照组：猪胶原膜（BG）；与HA相关的鱼胶原膜（CP）；与HA相关的牛胶原膜（CB），在术后7天、15天、30天和60天进行分析。在30天时，观察到CB组的膜完整性，而CP组和BG组的碎片分散在缺损中心。在60天时，BG组显示出更好的结果，与CP组无统计学差异（=0.199），与CB组有统计学显著差异（=0.013）。BG组和CP组的炎症特征相似。免疫组织化学显示，在60天时，BG组和CP组骨桥蛋白染色中等，CB组染色浅，BG组骨钙素染色强烈，而CB组和CP组染色中等。显微断层扫描显示，BG组的平均骨体积最高（14.247立方毫米），其次是CB组（11.850立方毫米）和CP组（9.560立方毫米）。与HA相关的胶原膜显示出骨促进潜力。
