Staines W A, Yamamoto T, Daddona P E, Nagy J I
Neurosci Lett. 1987 May 19;76(3):257-62. doi: 10.1016/0304-3940(87)90411-3.
Projections from the tuberomammillary nucleus (TM) to widespread regions within the hypothalamus were evaluated using a combined immunohistochemical-retrograde fluorescent tracing procedure. Injections of Fluoro-gold into the anterior hypothalamus labelled TM neurons identified by their immunoreactivity for the enzyme adenosine deaminase (ADA). Small injections of Fluoro-gold into the posterior hypothalamus also led to the labelling of TM neurons. The numbers of ADA-immunoreactive axonal varicosities were 5 times greater in the hypothalamus than in most other brain regions. The results indicate that the hypothalamus represents a major projection area of the TM.
使用免疫组织化学-逆行荧光追踪联合方法评估了结节乳头体核（TM）向下丘脑内广泛区域的投射。将荧光金注射到下丘脑前部，标记出对腺苷脱氨酶（ADA）具有免疫反应性的TM神经元。向下丘脑后部少量注射荧光金也导致TM神经元被标记。下丘脑内ADA免疫反应性轴突膨体的数量比大多数其他脑区多5倍。结果表明，下丘脑是TM的主要投射区域。
