Nagy J I, Buss M, Daddona P E
Neuroscience. 1986;17(1):141-56. doi: 10.1016/0306-4522(86)90232-0.
The localization and sources of adenosine deaminase-containing structures in the mesencephalic nucleus of the trigeminal nerve of the rat was studied using indirect immunofluorescence or immunoperoxidase immunohistochemical staining techniques for adenosine deaminase in combination with retrograde fluorescent tracing or lesion methods. The majority of large mesencephalic neurons were engulfed by a dense adenosine deaminase-immunoreactive plexus. Immunostaining was often punctate surrounding neuronal profiles or sometimes had the appearance of varicose fibers coursing over the neuronal surface. Occasionally, immunostained axons were found travelling towards and contacting mesencephalic neurons. Mesencephalic neuronal somas surrounded by immunofluorescence staining for adenosine deaminase were simultaneously labelled with fast blue after injections of this dye into the temporalis or masseter muscles of mastication. Injections of fast blue into the mesencephalic nucleus resulted in fast blue labelling of adenosine deaminase-immunoreactive neurons in the tuberal, caudal and postmammillary caudal magnocellular nuclei of the hypothalamus. Ablation of these hypothalamic nuclei caused a near total depletion of adenosine deaminase-immunostained fibers in the mesencephalic nucleus including those associated with mesencephalic neurons. It is concluded that adenosine deaminase-containing neurons in the posterior hypothalamus innervate mesencephalic primary sensory neurons, which are known to convey proprioceptive input to trigeminal motor nuclei controlling jaw muscles. The possibility is considered that the hypothalamus, via a direct action on these sensory neurons, may exert automatic control over jaw movements related to aggressive attack, defensive or feeding behavior. In addition, it appears that mesencephalic neurons may provide an ideal model system for electrophysiological investigations of the neurotransmitter(s) utilized by adenosine deaminase-containing hypothalamic projections.
采用间接免疫荧光或免疫过氧化物酶免疫组织化学染色技术检测大鼠三叉神经中脑核内含腺苷脱氨酶结构的定位和来源，并结合逆行荧光追踪或损伤方法进行研究。大多数中脑大神经元被密集的腺苷脱氨酶免疫反应性神经丛所包围。免疫染色通常围绕神经元轮廓呈点状，有时呈现为在神经元表面蜿蜒的曲张纤维外观。偶尔，可发现免疫染色的轴突朝着中脑神经元延伸并与之接触。在将快蓝注射到咀嚼肌颞肌或咬肌后，被腺苷脱氨酶免疫荧光染色包围的中脑神经元胞体同时被快蓝标记。将快蓝注射到中脑核内，导致下丘脑结节、尾侧和乳头体后尾侧大细胞核内的腺苷脱氨酶免疫反应性神经元被快蓝标记。切除这些下丘脑核会导致中脑核内腺苷脱氨酶免疫染色纤维几乎完全耗尽，包括那些与中脑神经元相关的纤维。得出的结论是，下丘脑后部含腺苷脱氨酶的神经元支配中脑初级感觉神经元，已知这些神经元将本体感觉输入传递到控制颌肌的三叉神经运动核。人们认为下丘脑可能通过对这些感觉神经元的直接作用，对与攻击、防御或进食行为相关的颌部运动施加自动控制。此外，中脑神经元似乎可能为研究含腺苷脱氨酶的下丘脑投射所利用的神经递质提供一个理想的电生理研究模型系统。
