Department of Chemistry and Chemical Biology , Harvard University , 12 Oxford Street , Cambridge , Massachusetts 02138 , United States.
Department of Chemistry , Clemson University , Clemson , South Carolina 29634 , United States.
Anal Chem. 2019 Jul 2;91(13):8298-8303. doi: 10.1021/acs.analchem.9b01020. Epub 2019 May 29.
This work describes a method to fabricate three-dimensional paper microfluidic devices in one step, without the need of stacking layers of paper, glue, or tape. We used a nontransparent negative photoresist that allows patterning selectively (vertically) the paper, creating systems of two or three layers, including channels. To demonstrate the capabilities of this methodology, we designed, fabricated, and tested a six-level diluter. The performance of the device was also simulated using a simple numerical model implemented in the program PETSc-FEM. The resulting μPAD is small (1.6 cm × 2.2 cm), inexpensive, requires low volumes of sample (5 μL), and is able to perform mixing and dilution in 2 min.
这项工作描述了一种一步制造三维纸微流控器件的方法，无需堆叠纸张、胶水或胶带。我们使用了一种不透明的负性光致抗蚀剂，允许选择性地（垂直）对纸张进行图案化，从而创建包括通道在内的两层或三层系统。为了展示这种方法的能力，我们设计、制造和测试了一个六级稀释器。该器件的性能也使用在 PETSc-FEM 程序中实现的简单数值模型进行了模拟。得到的 μPAD 体积小（1.6cm×2.2cm）、成本低、所需样品量少（5μL），并且能够在 2 分钟内完成混合和稀释。
