Wu-Wang C Y, Neu J
Prostaglandins Leukot Med. 1987 Apr;27(1):71-80. doi: 10.1016/0262-1746(87)90060-6.
This study was designed to compare prostaglandin (PG)-synthesizing activity in rat small intestinal microsomes in the 1st, 3rd, and 6th weeks of life and at maturity (greater than 100 days). When an incubation system was used containing 2 mg microsomal protein, 0.5 mM (-)-epinephrine and 1 mM reduced glutathione, the highest PG-synthesizing activity was achieved by incubating 0.157 mM 1-(14)C-arachidonate (specific activity 2.6 X 10(6) dpm/mumol) at 37 degrees C for 5 min. The labeled metabolites were extracted and then separated with high performance liquid chromatography. The four PGs analyzed were 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha), thromboxane B2, prostaglandin F2 alpha and prostaglandin E2. Enzymatic activity for the synthesis of 6-keto-PGF1 alpha was much higher than that for the other PGs. A significant difference was observed for the bioconversion from arachidonate to 6-keto-PGF1 alpha and total PGs among the four age groups of rats. The postweanling groups (week 6 and adult) showed significantly higher enzymatic activities for the syntheses of 6-keto-PGF1 alpha and total PGs than did the preweanling groups (weeks 1 and 3).
本研究旨在比较出生后第1周、第3周、第6周以及成年期（大于100天）大鼠小肠微粒体中前列腺素（PG）的合成活性。当使用含有2mg微粒体蛋白、0.5mM（-）-肾上腺素和1mM还原型谷胱甘肽的孵育体系时，通过在37℃孵育0.157mM 1-（14）C-花生四烯酸（比活度2.6×10（6）dpm/μmol）5分钟可达到最高的PG合成活性。标记的代谢产物经提取后，再用高效液相色谱进行分离。所分析的四种PG分别为6-酮-前列腺素F1α（6-酮-PGF1α）、血栓素B2、前列腺素F2α和前列腺素E2。6-酮-PGF1α的合成酶活性远高于其他PG。在四个年龄组的大鼠中，观察到花生四烯酸向6-酮-PGF1α和总PG的生物转化存在显著差异。断奶后组（第6周和成年）6-酮-PGF1α和总PG合成的酶活性显著高于断奶前组（第1周和第3周）。
