Kelly K L, Laychock S G
Prostaglandins. 1981 May;21(5):759-69. doi: 10.1016/0090-6980(81)90233-1.
Isolated pancreatic islets of Langerhans of the rat which were sonicated and incubated with radiolabeled arachidonic acid for 1 hr synthesized several species of prostaglandins (PGs). Both thin-layer and high-performance liquid (HPLC) chromatographic techniques demonstrated the synthesis by islet sonicates of PGF2 alpha and PGE2 equivalents, in addition to the 15-keto-13, 14-dihydro metabolites of these primary PGs. In addition, HPLC allowed the identification of 6-keto-PGF1 alpha (the metabolite of prostacyclin) as a major PG synthesized from arachidonate by this tissue. Islet vascular elements, as well as endocrine cells, may contribute to the synthesis of the latter compound. Lesser amounts of arachidonate were incorporated into PG-like compounds eluting as thromboxane. The synthesis of PGs was sensitive to the protein concentration of islet sonicate, and a five-fold dilution of protein resulted in a comparable reduction in arachidonate incorporation into PGs. Labeled arachidonate was also incorporated into compounds which elute as hydroxy or hydroperoxyeicosatetraenoic acids on HPLC. Thus, isolated pancreatic islets synthesize a variety of PGs which may have a physiological role in hormone secretion from this endocrine organ.
将大鼠分离的胰岛进行超声处理，并与放射性标记的花生四烯酸一起孵育1小时，结果显示其能合成多种前列腺素（PGs）。薄层色谱和高效液相色谱（HPLC）技术均表明，胰岛超声提取物除了能合成这些主要PGs的15-酮-13,14-二氢代谢物外，还能合成PGF2α和PGE2类似物。此外，HPLC鉴定出6-酮-PGF1α（前列环素的代谢物）是该组织由花生四烯酸合成的主要PG。胰岛血管成分以及内分泌细胞可能参与了后一种化合物的合成。较少数量的花生四烯酸被掺入以血栓素形式洗脱的PG样化合物中。PGs的合成对胰岛超声提取物的蛋白质浓度敏感，蛋白质浓度稀释五倍会导致花生四烯酸掺入PGs的量相应减少。标记的花生四烯酸也被掺入在HPLC上以羟基或氢过氧化二十碳四烯酸形式洗脱的化合物中。因此，分离的胰岛能合成多种PGs，这些PGs可能在此内分泌器官的激素分泌中发挥生理作用。
