Athinuwat Dusit, Brooks Siraprapa
Department of Agricultural Technology, Faculty of Science and Technology, Thammasat University, Pathumthani, Thailand.
School of Science, Mae Fah Luang University, Chaing Rai, 57100, Thailand.
Curr Microbiol. 2019 Jul;76(7):879-887. doi: 10.1007/s00284-019-01702-y. Epub 2019 May 14.
The goal of this study was to elucidate the role of the outer membrane protein A (ompA) gene of Xanthomonas axonopodis pv. glycines in bacterial pustule pathogenesis of soybean. An ompA mutant of X. axonopodis pv. glycines KU-P-SW005 was shown to significantly decrease cellulase, pectate lyase, and polysaccharide production. The production of these proteins in the ompA mutant was approximately five times lower than that of the wildtype. The ompA mutant also exhibited modified biofilm development. More importantly, the mutant reduced disease severity to the soybean. Ten days after inoculation, the virulence rating of the susceptible soybean cv. SJ4 inoculated with the ompA mutant was 11.23%, compared with 87.98% for the complemented ompA mutant. Production of cellulase, pectate lyase, polysaccharide was restored, biofilm, and pustule numbers were restored in the complemented ompA mutant that did not differ from the wild type. Taken together, these data suggest that OmpA-mediated invasion plays an important role in protein secretion during pathogenesis to soybean.
本研究的目的是阐明大豆细菌性斑点病菌野油菜黄单胞菌大豆致病变种（Xanthomonas axonopodis pv. glycines）外膜蛋白A（ompA）基因在大豆细菌性斑点病发病机制中的作用。野油菜黄单胞菌大豆致病变种KU-P-SW005的ompA突变体显示出纤维素酶、果胶酸裂解酶和多糖产量显著降低。ompA突变体中这些蛋白质的产量比野生型低约五倍。ompA突变体还表现出生物膜形成的改变。更重要的是，该突变体降低了对大豆的病害严重程度。接种十天后，接种ompA突变体的感病大豆品种SJ4的毒力评级为11.23%，而互补的ompA突变体为87.98%。互补的ompA突变体中纤维素酶、果胶酸裂解酶、多糖的产量得以恢复，生物膜和病斑数量也恢复到与野生型无差异的水平。综上所述，这些数据表明OmpA介导的入侵在大豆发病过程中的蛋白质分泌中起重要作用。
