School of Life Sciences, Nantong University, Nantong, P. R. China.
Centro Nacional de Biotecnología, CSIC, Madrid, Spain.
Pest Manag Sci. 2021 Apr;77(4):1668-1673. doi: 10.1002/ps.6186. Epub 2020 Nov 29.
Xanthomonas axonopodis pv. glycines (Xag) is a hazardous pathogen able to cause bacterial pustule disease in soybean, reducing crop yield and quality. Although flavonoids rutin and genistein are known to play an important role in soybean defence, soybean is only able to produce Biochanin A in low concentration.
In this work, Biochanin A was found to produce higher antibacterial activity against Xag in comparison with genistein (minimum inhibitory concentration < 100 μg/mL). Biochanin A was able to inhibit DNA synthesis and flagella formation in Xag, and altered the composition of the bacterial membrane. These effects reduced swimming motility, extracellular protease activity and biofilm formation. Further, Biochanin A was tested for the control of Xag in soybean leaves, showing similar, or even higher, inhibitory ability in comparison with some products commonly used for the control of this pathogen.
The antibacterial properties of Biochanin A against Xag have been studied for the first time, revealing new insights on the potential applications of this isoflavonoid for the management of bacterial pustule disease. © 2020 Society of Chemical Industry.
野油菜黄单胞菌（Xag）是一种危险的病原体，能够引起大豆细菌性斑疹病，降低作物产量和质量。虽然已知类黄酮芦丁和染料木黄酮在大豆防御中起着重要作用，但大豆只能产生低浓度的大豆素 A。
本工作发现大豆素 A 对 Xag 的抑菌活性高于染料木黄酮（最小抑菌浓度<100μg/mL）。大豆素 A 能够抑制 Xag 的 DNA 合成和鞭毛形成，并改变细菌膜的组成。这些作用降低了泳动性、胞外蛋白酶活性和生物膜形成。此外，还在大豆叶片中测试了大豆素 A 对 Xag 的控制作用，与一些常用的控制该病原体的产品相比，其具有相似甚至更高的抑制能力。
首次研究了大豆素 A 对 Xag 的抑菌特性，为该异黄酮类化合物在细菌性斑疹病防治中的潜在应用提供了新的见解。 © 2020 英国化学学会。
