Yunnan Institute of Food Safety , Kunming University of Science and Technology , Kunming , Yunnan 650500 , People's Republic of China.
College of Chemical and Environmental Engineering, Key Laboratory of Low Carbon Energy and Chemical Engineering , Shandong University of Science and Technology , Qingdao , Shandong 277590 , People's Republic of China.
J Agric Food Chem. 2019 Jun 5;67(22):6248-6256. doi: 10.1021/acs.jafc.9b01208. Epub 2019 May 24.
A lignin amphoteric surfactant and betaine could enhance the enzymatic hydrolysis of lignocellulose and recover cellulase. The effects of lignosulfonate quaternary ammonium salt (SLQA) and dodecyl dimethyl betaine (BS12) on enzymatic hydrolysis digestibility, ethanol yield, yeast cell viability, and other properties of high-solid enzymatic hydrolysis and fermentation of a corncob residue were studied in this research. The results suggested that SLQA and 1 g/L BS12 effectively improved the ethanol yield through enhancing enzymatic hydrolysis. SLQA had no significant effect on the yeast cell membrane and glucose fermentation. However, 5 g/L BS12 reduced the ethanol yield as a result of the fact that 5 g/L BS12 damaged the yeast cell membrane and inhibited the conversion of glucose to ethanol. Our research also suggested that 1 g/L BS12 enhanced the ethanol yield of corncob residue fermentation, which was attributed to the fact that lignin in the corncob adsorbed BS12 and decreased its concentration in solution to a safe level for the yeast.
一种木质素两性表面活性剂和甜菜碱可以提高木质纤维素的酶水解效率并回收纤维素酶。本研究考察了木质素磺酸钠季铵盐（SLQA）和十二烷基二甲基甜菜碱（BS12）对玉米芯残渣高固体酶水解和发酵过程中酶水解消化率、乙醇产量、酵母细胞活力和其他性能的影响。结果表明，SLQA 和 1 g/L BS12 通过提高酶水解作用有效地提高了乙醇产量。SLQA 对酵母细胞膜和葡萄糖发酵没有显著影响。然而，5 g/L BS12 降低了乙醇产量，因为 5 g/L BS12 破坏了酵母细胞膜并抑制了葡萄糖向乙醇的转化。我们的研究还表明，1 g/L BS12 提高了玉米芯残渣发酵的乙醇产量，这归因于木质素在玉米芯上吸附了 BS12，从而降低了其在溶液中的浓度，使其对酵母处于安全水平。
