Key Laboratory of Chemical Biology of Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, China.
Chemistry. 2019 Jul 22;25(41):9629-9633. doi: 10.1002/chem.201902222. Epub 2019 Jun 27.
We here report a simple and efficient strategy of fabricating artificial multi-enzyme complex (MEC) based on the integration of natural enzyme with catalytic compartment. As a proof of concept, this strategy was demonstrated by selecting cholesterol oxidase (ChOx) and Ce -based nanoscale coordination polymer (Ce-NCP) with peroxidase-like activity as the models, which forms ChOx@Ce-NCP. Benefitting from the confinement and sheltering effects of Ce-NCP, superior cascade activity and stability in harsh environments were achieved in ChOx@Ce-NCP. Meanwhile, the distinct advantage of ChOx@Ce-NCP has also been highlighted by its negligible substrate inhibition effect and adjustable mass ratio of building blocks. Upon the doping of Tb in ChOx@Ce-NCP, a luminescent artificial MEC (ChOx@Ce-NCP:Tb) was further fabricated to drive a cascade amplified time-resolved fluorescent assay within a confined space, showing high sensitivity and specificity toward cholesterol.
我们在此报告了一种基于天然酶与催化隔室集成的人工多酶复合物（MEC）的简单而有效的构建策略。作为概念验证，我们选择胆固醇氧化酶（ChOx）和具有过氧化物酶样活性的 Ce 基纳米级配位聚合物（Ce-NCP）作为模型，构建了 ChOx@Ce-NCP。受益于 Ce-NCP 的限制和保护作用，在 ChOx@Ce-NCP 中实现了优越的级联活性和在恶劣环境下的稳定性。同时，通过掺杂 Tb，进一步构建了发光人工 MEC（ChOx@Ce-NCP：Tb），可在受限空间内驱动级联放大时间分辨荧光分析，对胆固醇具有高灵敏度和特异性。
