Weiler H, von Bülow V
Vet Immunol Immunopathol. 1987 Mar;14(3):257-67. doi: 10.1016/0165-2427(87)90094-8.
Chicken thymus, spleen, and bursa lymphocytes were isolated by different methods and incubated under differing conditions in order to obtain and characterize avian lymphokines. The biological activity of lymphokine-containing cell culture supernatants was measured by their antiviral activity (interferon(IFN)-units) and by their capacity to induce cytostatic effects in bone-marrow-derived macrophages (50% cytostasis-inducing dose, CID). Lymphokine production by thymus lymphocytes required concanavalin A (ConA)-stimulation, while spleen cells, when cultured at high density, released CID and IFN activities into the culture medium even without mitogen-stimulation. By way of comparison, the highest lymphokine content was found in the supernatant of lymphocyte cultures, which were incubated for 72 hours at 41 degrees C after stimulation with an optimal ConA dose. For stimulation of thymus lymphocytes 30 micrograms ConA/ml were found to be optimal, independent of serum content and cell density in the cultures. In contrast, the optimal ConA dose for spleen lymphocytes not only depended on the serum content but also on the cell density in the cultures and varied within a range of 2.5 micrograms and 45 micrograms ConA/ml.
通过不同方法分离鸡的胸腺、脾脏和法氏囊淋巴细胞，并在不同条件下进行培养，以获得禽淋巴因子并对其进行特性分析。含淋巴因子的细胞培养上清液的生物活性通过其抗病毒活性（干扰素（IFN）单位）以及在骨髓来源巨噬细胞中诱导细胞生长抑制效应的能力（50%细胞生长抑制剂量，CID）来测定。胸腺淋巴细胞产生淋巴因子需要伴刀豆球蛋白A（ConA）刺激，而脾脏细胞在高密度培养时，即使没有丝裂原刺激也会向培养基中释放CID和IFN活性。相比之下，在淋巴细胞培养上清液中发现最高的淋巴因子含量，这些上清液在用最佳ConA剂量刺激后于41℃孵育72小时。对于胸腺淋巴细胞的刺激，发现30微克ConA/毫升是最佳的，与培养物中的血清含量和细胞密度无关。相比之下，脾脏淋巴细胞的最佳ConA剂量不仅取决于血清含量，还取决于培养物中的细胞密度，并且在2.5微克至45微克ConA/毫升的范围内变化。
