Wilson C B, Westall J, Johnston L, Lewis D B, Dower S K, Alpert A R
J Clin Invest. 1986 Mar;77(3):860-7. doi: 10.1172/JCI112383.
Human neonatal lymphocytes produced little macrophage activation factor in response to mitogens. This correlated with decreased production of interferon-gamma (IFN gamma): adult lymphokines contained 894.2 +/- 177.1 U/ml, whereas neonatal cord and peripheral lymphokines contained 66.9 +/- 17.0 and 116.7 +/- 29.6 U/ml by bioassay. Results by radioimmunoassay (RIA) for IFN gamma were similar. In contrast, the interleukin 2 content of cord lymphokines was greater (P less than 0.01) and that of neonatal peripheral blood lymphokines similar to that of adults. Interleukin 1 production and interleukin 2 receptor expression and affinity were similar for adult and neonatal cells. Interleukins 1 and 2 in amounts comparable to those in adult lymphokines did not increase production of macrophage activation factor or IFN gamma by neonatal cells. Neonatal cells did not contain intracellular IFN or degrade exogenous IFN. Excess suppressor activity was not found in neonatal cultures. Addition of IFN alpha, 10,000-50,000 U/ml of interleukin 2 or phorbol myristate acetate (PMA) to cord mononuclear cells or of adult monocytes or PMA to cord T cells increased IFN gamma production compared to cells stimulated with concanavalin A (ConA) alone. Nevertheless, under optimal conditions (T cells + PMA + Con A), adult cells produced much more IFN gamma (1,360 +/- 261 U/ml by RIA) than cord cells (122 +/- 37 U/ml). Staphylococcal enterotoxin A (SEA) stimulated cord cell IFN gamma production at low cell densities; nevertheless, adult cells produced more IFN in response to SEA 1,341 +/- 350 U/ml) than cord cells (350 +/- 33 U/ml). Decreased production of IFN gamma by neonatal cells appears to be due both to differences in their intrinsic capacity to produce IFN gamma and to differences in regulatory mechanisms.
人类新生儿淋巴细胞对有丝分裂原产生的巨噬细胞活化因子很少。这与干扰素-γ（IFNγ）产量降低相关：通过生物测定法，成人淋巴因子中IFNγ含量为894.2±177.1 U/ml，而新生儿脐带血和外周血淋巴因子中分别为66.9±17.0和116.7±29.6 U/ml。IFNγ的放射免疫测定（RIA）结果相似。相比之下，脐带血淋巴因子中白细胞介素2含量更高（P<0.01），新生儿外周血淋巴因子中白细胞介素2含量与成人相似。成人和新生儿细胞的白细胞介素1产生、白细胞介素2受体表达及亲和力相似。与成人淋巴因子中含量相当的白细胞介素1和白细胞介素2并不能增加新生儿细胞产生巨噬细胞活化因子或IFNγ的量。新生儿细胞不含细胞内IFN，也不降解外源性IFN。在新生儿培养物中未发现过多的抑制活性。与仅用伴刀豆球蛋白A（ConA）刺激的细胞相比，向脐带血单个核细胞中添加10000 - 50000 U/ml的IFNα、白细胞介素2或佛波酯肉豆蔻酸酯（PMA），或向脐带血T细胞中添加成人单核细胞或PMA，均可增加IFNγ的产生。然而，在最佳条件下（T细胞+PMA+ConA），成人细胞产生的IFNγ（通过RIA测定为1360±261 U/ml）比脐带血细胞（122±37 U/ml）多得多。葡萄球菌肠毒素A（SEA）在低细胞密度时刺激脐带血细胞产生IFNγ；然而，成人细胞对SEA产生的IFN（1341±350 U/ml）比脐带血细胞（350±33 U/ml）多。新生儿细胞产生IFNγ减少似乎是由于其产生IFNγ的内在能力差异以及调节机制差异所致。
