Havill A M, Gee M H
Am J Pathol. 1987 Jun;127(3):441-6.
The rate and sequence of interstitial and alveolar fluid removal from the lung after the occurrence of pulmonary edema were examined. Rats were given intraperitoneal injections of 20 mg/kg alpha-naphthylthiourea (ANTU), resulting in an increased permeability edema with alveolar flooding. Animals were killed at intervals between 2 and 48 hours after ANTU for the gravimetric determination of extravascular lung water (Qwl/dQl) and histologic study of the lung. Interstitial fluid volume was quantified by a morphometric technique. The assumptions were made that edema fluid equaled the experimental Qwl/dQl minus the normal Qwl/dQl, and that the edema fluid volume equaled the sum of interstitial and alveolar fluid volume. It was found that between 2 and 4 hours after the induction of pulmonary edema, fluid was removed from the alveolar space faster than it was removed from the interstitial space. Between 4 and 48 hours after ANTU, the fluid removal rate from both compartments was much slower, and interstitial fluid was removed at a faster rate than alveolar fluid. It is hypothesized that the later phase of fluid removal from the lung is dependent on the removal of protein.
研究了肺水肿发生后肺间质和肺泡液清除的速率及顺序。给大鼠腹腔注射20mg/kg的α-萘基硫脲（ANTU），导致通透性增加性肺水肿并伴有肺泡灌洗。在ANTU注射后2至48小时的不同时间点处死动物，用于重量法测定血管外肺水（Qwl/dQl）并对肺进行组织学研究。采用形态计量技术对间质液体积进行量化。假设水肿液等于实验性Qwl/dQl减去正常Qwl/dQl，且水肿液体积等于间质液和肺泡液体积之和。结果发现，在肺水肿诱导后2至4小时内，肺泡腔内液体的清除速度比间质内液体的清除速度快。在ANTU注射后4至48小时，两个腔室的液体清除速度都慢得多，且间质液的清除速度比肺泡液快。据推测，肺内液体清除的后期阶段取决于蛋白质的清除。
