Sobiech K A, Karpiak S E
Arch Immunol Ther Exp (Warsz). 1986;34(5-6):541-6.
Glycogen-protein complexes from the muscles of Celerio euphorbiae and horse as well as from the livers of frog, carp and man were isolated and used as antigens. After immunization of rabbits antisera were obtained from which fractions of immunoglobulins were isolated. Antibodies were used in agar immunodiffusion tests and in quantitative reaction of immunoprecipitation with glycogens. The immunoprecipitation test proved the occurrence of quantitative differences in cross reaction of complexes with different origin antibodies, testifying to variability of their antigen properties. The immunodiffusion test allows to suppose the presence in antigens of at least two determinants of protein-carbohydratic character.
从甘薯天蛾和马的肌肉以及青蛙、鲤鱼和人的肝脏中分离出糖原 - 蛋白质复合物，并将其用作抗原。用这些抗原免疫兔子后获得抗血清，再从抗血清中分离出免疫球蛋白组分。将抗体用于琼脂免疫扩散试验以及与糖原的免疫沉淀定量反应中。免疫沉淀试验证明，不同来源复合物与抗体的交叉反应存在定量差异，这证明了它们抗原性质的可变性。免疫扩散试验表明，抗原中至少存在两种具有蛋白质 - 碳水化合物性质的决定簇。
